SPE-HPLC分析全血中37种药(毒)物  

Analysis of 37 Drugs in Whole Blood by HPLC after Solid Phase Extraction

作　　者：梁晨 张玉荣 金琦芸[2] 郭幼梅[2] 

机构地区：[1]上海市刑事科学技术研究所,上海200083 [2]复旦大学药学院药物分析教研室,上海200032

出　　处：《法医学杂志》2006年第5期349-352,共4页Journal of Forensic Medicine

摘　　要：目的建立全血中37种常见药(毒)物的固相萃取-高效液相色谱(SPE-HPLC)分析方法。方法以多沙普仑为内标,0.5mL全血经Oasis小柱固相萃取后,用HPLC进行分析,内源性物质不干扰测定。色谱柱采用LiChrospher!100RP-C18柱(250mm×4.0mm×5μm);二极管阵列检测器,检测波长为230nm和250nm,同时进行紫外扫描;柱温50℃。结果37种药(毒)物的绝对回收率除吗啡外均大于61.68%;日内及日间精密度均小于10%;检测限1～30ng/mL;线性相关系数在0.99798以上。结论本法快速、灵敏、重现性好,可用于实际案例中多种药(毒)物的分析。Objective To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood. Methods With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 （250mm×4.0mm×5μm）column kept at 50℃, the DAD detector was set at 230nm and 250nm. Results The limit of detection were 1-30ng/mL. The method showed excellent linearity and the linear correlation coefficient was≥0.997 98. The relative standard deviation for between-day and within-day assay were 〈10%. Conclusion The method is effective, simple, reliable and has been used in real cases.

关 键 词：固相萃取 高效液相色谱法 全血 

分 类 号：R914[医药卫生—药物化学]