上皮型钙黏附蛋白、CD44v6及连接蛋白43在肝细胞癌中的表达及意义  

Expression of epithelial-cadherin,CD44v6 and connexin43 in hepatocellular carcinoma

作　　者：张宝燕[1] 戴晓汶[1] 陈清勇[2] 方丽[1] 钱斌[1] 孙国英[1] 崔海宏 

机构地区：[1]解放军第一一七医院病理科,杭州310013 [2]解放军第一一七医院呼吸科,杭州310013 [3]解放军第四五六医院内四科

出　　处：《中华病理学杂志》2006年第10期616-619,共4页Chinese Journal of Pathology

摘　　要：目的研究上皮型钙黏附蛋白(E-cad)、CD44v6和连接蛋白43(Cx43)在人肝细胞癌(HCC)中的表达及其表达与患者性别、年龄和组织学分级的关系。方法采用免疫荧光双标记染色技术结合激光扫描共聚焦显微镜观察 E-cad、CD44v6及 Cx43在30例正常肝组织,25例肝良性病变和38例 HCC 中的表达;另在 HCC 组检测并分析了该3种标记物的表达与性别、年龄和组织学分级的关系。结果 E-cad 与 Cx43均在正常肝组织及肝良性病变组高表达,而在 HCC 组表达都明显降低,前两组与 HCC 组间表达强度差异均有统计学意义(F_(E-cad)=879.2,F_(Cx43)=303.7,P<0.05)。相反,CD44v6在正常肝组织及肝良性病变组表达较低,而在 HCC 中表达较高,差异有统计学意义(F=2057.2,P<0.05)。HCC 组 E-cad 和 Cx43阳性表达强度在患者不同性别、年龄及肿瘤的组织学分级间的差异均无统计学意义(P>0.05);而 CD44v6表达与 HCC 的组织学分级有关,HCC 分化差,CD44v6表达高(t=-2.06,P<0.05),但不同年龄、性别组 HCC 的 CD44v6阳性表达强度差异无统计学意义(P>0.05)。HCC 组 E-cad 与 Cx43的表达呈正相关,而 E-cad、Cx43与 CD44v6的表达呈负相关。结论 HCC 的发生、发展伴随着多种分子表型的改变。E-cad、Cx43的低表达与 CD44v6的高表达可能参与 HCC 的侵袭,尤其是 CD44v6的表达还与 HCC 的组织学分级相关。三者联合检测对判断HCC 的诊断和预后有一定价值。Objective To study the expression of epithelial-cadherin （E-cad）, CD44v6 and Cx43 in hepatoeellular carcinoma （ HCC ） and its relationship with sex and age of patients, as well as tumor histopathologie grades. Methods Double immunofluorescent staining and laser scanning eonfoeal microscopy was used to study the expression of E-cad, CD44v6 and Cx43 in 30 cases of normal liver tissue, 25 cases of benign hepatic lesions and 38 cases of HCC. In the HCC group, correlation of antigen expression with sex and age of patients and tumor histopathologie grades was studied by T-test. Results Significant decrease in expression of E-cad and Cx43 was noted in HCC group, as compared to normal liver tissue and benign hepatic lesion （ P 〈 0. 05 ）. On the other hand, CD44v6 expression was higher in HCC group than in the other two groups （P 〈 0.05）. In HCC group, the expression of E-cad and Cx43 did not correlate with sex, age and histopathologie grades （ P 〉 0. 05 ）. However, CD44v6 expression positively correlated with higher tumor histopathologie grades （ P 〈 0. 05 ） but not sex and age of patients （ P 〉 0. 05 ）. In HCC group, the expression of E-cad positively correlated with that of Cx43, while the expression of CD44v6 negatively correlated with that of E-cad and Cx43. Conclusions Tumor immunophenotype alters during development and progression of HCC. Low expression of E-cad and Cx43 and high expression of CD44v6 may be related to aggressive clinical behavior of HCC, moreover, high expression of CD44v6 correlated with high tumor grades. Detection of E-cad, CD44v6 and Cx43 expression may thus be useful in predicting prognosis of HCC.

关 键 词：癌 肝细胞 显微镜检查 共焦 荧光抗体技术 连接蛋白类 

分 类 号：R735.7[医药卫生—肿瘤]