核因子κB、Ki-67和MMP-9在牙源性钙化囊肿中的表达  

Expression of nuclear factor-kB,Ki-67 and matrix metalloproteinase-9 in calcifying odontogenic cyst

作　　者：巩艳玲[1] 王丽[2] 陈新明[2] 王恒琨[1] 王新红[2] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室 [2]武汉大学口腔医学院病理科,430079

出　　处：《中华口腔医学杂志》2006年第10期627-630,共4页Chinese Journal of Stomatology

摘　　要：目的检测核因子κB(NF-κB)、Ki-67和基质金属蛋白酶9(MMP-9)在牙源性钙化囊肿(COC)中的表达,探讨 COC 的增殖活性和侵袭性。方法根据2005年 WHO 关于牙源性肿瘤的分类标准,将26例被诊断为 COC 的病例分为牙源性钙化囊性瘤(calcifying cystic odontogenic tumor,CCOT)、成牙本质影细胞瘤(dentinogenic ghost cell tumor,DGCT)、牙源性影细胞癌(ghost cellodontogenic carcinoma,GCOC)3种病损,用免疫组化方法检测 NF-κB(p65)、Ki-67、MMP-9在 COC 和10例一般型成釉细胞瘤中的表达。结果 NF-κB主要在肿瘤细胞胞质中表达,偶见胞核表达(核阳性率<1%)。Ki-67在 GCOC 中的表达明显高于 CCOT(P<0.001)、DGCT(P<0.05)和成釉细胞瘤(P<0.005),差异有统计学意义。MMP-9在肿瘤细胞及间质细胞中均有表达,GCOC 间质细胞 MMP-9的阳性表达率明显高于其余3组良性牙源性肿瘤(P<0.05)。结论 NF-κB在 COC 的恶性转化及侵袭过程中影响较小。GCOC 的增殖活性和侵袭性明显高于 CCOT 和 DGCT,GCOC 间质中的 MMP-9是其高侵袭性的重要原因。Objective To evaluate the expression of nuclear factor-gB （NF-κB）, Ki-67 and matrix metalloproteinase-9 （MMP-9） in calcifying odontogenic cyst （COC）, in order to investigate the proliferation and invasion of COC. Methods Twenty-six cases of COC were classified into calcifying cystic odontogenic tumor （CCOT）, dentinogenic ghost cell tumor （DGCT） and ghost cell odontogenic carcinoma （GCOC） based on the WHO classification of odontogenic tumors in 2005. The specimens of COC and 10 classic ameloblastoma（AB） were examined immunohistochemically to determine the expression of NF-κB p65, Ki-67 and MMP-9. Results NF-κB was mainly detected in the cytoplasm of most tumor cells, but was only detected in the nucleus of few tumor cells （ rate of nuclear staining 〈 1% ）. The expression of Ki-67 was significantly higher in GCOC than in CCOT（P 〈 0. 001 ）, DGCT（P 〈 0. 05 ） and AB （ P 〈 0. 005 ）. MMP-9 was detected both in tumor cells and stromal cells. GCOC showed significantly higher percentage of MMP-9 positive cases in stromal cells than CCOT, DGCT and AB （P 〈 0.05 ）. Conclusions NF-κB may minimally affect the progression and invasion of COC. GCOC shows significantly higher proliferative activity and aggressiveness than CCOT and DGCT. MMP-9 in stroma may play a key role in the invasion of GCOC.

关 键 词：牙源性囊肿 钙化 NF—κB 基质金属蛋白酶 

分 类 号：R782[医药卫生—口腔医学]