利用毛细管电泳技术分析云南回族人群7个STR基因座的遗传多态性  

Genetic polymorphism of 7 STR loci in Hui population of Yunnan province by capillary electrophoresis

作　　者：呙恒娟[1] 文富强[1] 应斌武[1] 刘代顺[1] 邱容[1] 陈亚娟[1] 徐丹[1] 

机构地区：[1]四川大学华西医院呼吸内科,成都610041

出　　处：《第三军医大学学报》2006年第21期2119-2122,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金杰出青年基金资助项目(30425007);国家自然科学基金资助项目(30370627);美国纽约CMB基金资助项目;中国博士后基金资助项目(2005038268)~~

摘　　要：目的对云南回族群体的D3S1358/THO1/D21S11/D18S51/D5S818/D13S317/D7S820等7个STR基因座的遗传多态性进行群体遗传学研究。方法利用荧光标记复合扩增及毛细管电泳自动荧光检测的方法,对204例无关个体的7个STR基因座进行检测。结果获得7个基因座的各基因座等位基因的分布频率和基因型频率,结果均符合Hardy-Weinberg平衡。计算了各基因座的杂合度(He)、个人识别能力(PD)、偶合率(PM)、非父排除率(PE)和多态性信息总量(PIC)等群体遗传学数据。结论利用毛细管电泳技术可对这7个STR基因座进行很好的检测,这些基因座多态性丰富、灵敏度高,可用于人类遗传分析及法医学中的亲子鉴定和个人识别。Objective To investigate the allele frequency of seven short tandem repeats （STR） loci： D3 S 1358/THO 1/D21 S 11/D 18S51/D5 S818/D 13 S317/D7 S820 in Hui population of Yunnan province. Methods Totally 204 unrelated individuals from Hui population in Yunnan province were inspected for 7 STR polymorphism D3S1358/THO1/D21Sll/D18SS1/DSSS18/D13S317/DTS820 by means of capillary electrophoresis and fluorescence multiple amplification. Results The genotype distributions of each locus were in accordance with Hardy-Weinberg equilibrium. Some population genetic data, including the measured values of heterozygosity, the polymorphism information content （PIC）, the discrimination power （PD）, the power of exclusion （PE） were calculated. Conclusion Our results show that 7 STR loci are valuable STR loci genetic markers and useful in human genetics and forensic medicine.

关 键 词：毛细管电泳 短串联重复序列(STR) 复合扩增 群体遗传学 

分 类 号：R394.5[医药卫生—医学遗传学] R446.9[医药卫生—基础医学]