慢性增强型体外反搏对高胆固醇血症猪胸主动脉内皮细胞蛋白质表达的影响  

Effects of chronic EECP on protein expression in thoracic aorta endothelial cells in porcine with hypercholesteremia

作　　者：陈小林[1] 何小洪[1] 张焰[1] 钱月桃[2] 梁陆光[2] 方典秋[2] 詹澄扬[2] 郑振声[2] 马虹[1] 王奎建[3] 

机构地区：[1]中山大学附属第一医院心内科,广东广州510080 [2]中山大学卫生部辅助循环重点实验室,广东广州510080 [3]中山大学基础学院生物医学工程系,广东广州510080

出　　处：《中国病理生理杂志》2006年第10期1939-1942,共4页Chinese Journal of Pathophysiology

基　　金：国家"十五"科技攻关项目资助(No.2001BA706B07);国家自然科学基金资助(No.30127001)

摘　　要：目的:利用蛋白质组学方法探讨慢性EECP对高胆固醇血症猪胸主动脉内皮细胞(ECs)蛋白表达的影响。方法:高胆固醇血症猪反搏36 h后,收集胸主动脉ECs进行蛋白质组学检查。结果:与高脂对照组相比,在反搏组多检测到6种蛋白质。结论:慢性EECP调节动脉ECs蛋白表达,通过改善ECs粘附性和脂质代谢及减轻ECs凋亡拮抗高胆固醇脂血症对动脉内皮的损伤作用。AIM： To explore the effect of chronic enhanced external counterpulsation （EECP） on the protein expression in thoracic aorta endothelial cells in porcine with hypercholesteremia by proteomic techniques. METHODS： After performed EECP for 36 h on the hypercholesteremia porcines, the endothelial cells （ECs） were collected from the thoracic aorta and analyzed with proteomic techniques. RESULTS ： Six kinds of protein were detected in ECs in the EECP group, but not in the hypercholesteremia control group. CONCLUSION： Chronic EECP may protect ECs against damage resulted from hypercholesteremia by regulating protein expression in artery ECs, subsequently improving cell adhesion and intracellular lipid metabolism and reducing ECs apoptosis.

关 键 词：反搏动术 高胆固醇血症 内皮细胞 蛋白质组 

分 类 号：R363[医药卫生—病理学]