3个S100A基因在胃癌及其正常黏膜中表达的QRT-PCR检测  

Expression of three S100A calcium-binding proteins in human gastric cancer and non-cancerous mucosa as detected by QRT-PCR

作　　者：黄海力[1] 吴本俨[1] 朱旭东[2] 尤纬缔[1] 王孟薇[1] 

机构地区：[1]解放军总医院南楼消化科,北京100853 [2]军事医学科学院生物工程研究所

出　　处：《解放军医学杂志》2006年第10期947-950,共4页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十五"计划基金重点课题(01Z035)

摘　　要：目的比较S100A2、S100A4、S100A6基因在胃癌及其对应正常黏膜中的表达情况。方法应用实时定量RT-PCR构建上述3个基因的定量扩增标准曲线,精确定量3个基因在20例配对胃癌中的表达丰度并分析它们与胃癌的相关性。结果S100A2、S100A4、S100A6基因相对于β2微球蛋白(β2-MG)基因的表达丰度分别为2·83×10-4、6·44×10-2、0·41,3个基因在胃癌中表达均升高,升高倍数分别为10·78、2·31和2·25倍。与正常黏膜比较,S100A2、S100A6表达显著升高(P<0·05)。Spearman分析显示,S100A2与S10A4、S100A2与S100A6基因表达之间呈正相关(P值分别为0·00和0·017)。结论S100A2、S100A6基因表达变化可能是胃癌的早期事件,S100A4基因则可能与胃癌的浸润密切相关。Objective To verify the authors＇ previous cDNA micro-array results and to further investigate the moleculer mechanisms of gastric cancer. Methods Quantitative real time RT-PCR was employed to detect the expressions of three S100A calcium-binding genes in 22 fresh surgical samples of gastric tumor tissue and non-cancerous mucosa from the same patients. Results The transcription level of S100A2 in primary cancer lesion was elevated in 80% of samples when compared with matching non-neoplastic mueosa （P=0. 018） and the average up-regulation level was 10. 78 fold. 550% of cancer lesions showed higher transcription level of S100A4 than their adjacent non-neoplastic mucosa, the average up-regulation level was 2. 31 fold. S100A6 transcription level was higher in 740/00 （P=0. 01） of primary cancer lesion with an 2. 25 fold up-regulation than the adjacent non-neoplastic mucosa. After rectified by β2-microglobulin, the relative expression levels of S100A2, S100A4 and S100A6 were 2. 83 × 10^-4 , 6. 44 ×10^-2 and 0. 41, respectively. According to the Spearman correlation coefficient analysis there were significant positive correlations between S100A2 and S100A4, and S100A2 and S1006 （P value were 0. 00 and 0. 017, respectively）. Conclusion The changes in S100A2 and S100A6 genes may be an early event in a majority of gastric cancer patients, while S100A4 may be associated with the infiltration of gastric cancer. Further study on the three genes might be helpful for understanding the nature of gastric carcinoma.

关 键 词：实时定量RT-PCR S100A钙结合蛋白 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]