^(18)F-FLT在肺癌模型小鼠体内的生物分布及PET显像研究  

Experimental study on biodistribution and PET imaging of 3′-deoxy-3′-^(18)F-fluorothymidine in murine model of lung carcinoma

作　　者：柳曦[1] 周乃康[1] 张锦明100853北京 梁朝阳[1] 郑昕[1] 田嘉禾100853北京 

机构地区：[1]解放军总医院胸外科,北京100853 [2]解放军总医院核医学科

出　　处：《解放军医学杂志》2006年第10期960-962,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军"十一五"医药卫生科研基金资助项目(06MA241)

摘　　要：目的评价3′-脱氧-3′-18F-氟代胸苷(18F-FLT)作为一种新的PET示踪剂在恶性肿瘤诊断中的作用。方法采用荷肺腺癌T739小鼠模型。实验组经尾静脉注入18F-FLT后30、60、90、120min(每时间点8只小鼠)分别用井型探测仪测量18F-FLT的生物分布,并行PET显像。对照组(n=8)小鼠在注入18F-FDG后60min测量生物分布并行PET显像。结果18F-FLT在肿瘤部位有相当高的放射性摄取,在肾脏和脾脏亦有很高的放射性,肿瘤对血、肌肉及肺的T/NT比值均大于2。应用18F-FLT的肿瘤PET显像清晰。结论肺腺癌组织中18F-FLT摄取高于正常组织,可通过PET清晰显像,为进一步的临床研究提供了依据。Objective To investigate the biodistribution and positron emission tomography （PET） imaging of 3＇-deoxy-3＇-^18 F-fluorothymidine （^18F-FLT） in a murine model of pulmonary carcinoma, and to evaluate the use of ^18F-FLT as a new PET tracer for diagnosis of pulmonary malignant tumor. Methods 40 T739 mice bearing the pulmonary adenocarcinoma were randomly divided into five groups according to different tracers and time after their injection （n=8/group）. The biodistribution of mice for ^18F-FLT was measured with wellgamma detector at 30min, 60min, 90min, 120min after injection via the tail veins. The biodistribution of mice for ^18F-FDG was examined at 60min after injection as controls. In addition, the PET imaging of mice was performed using two tracers. Results In the biodistribution study of ^18F-FLT, considerable radioactive uptake in tumor was observed, and high radioactivity was showed in the kidney and spleen. The T/NT ratios of tumor/blood, tumor/muscle and tumor/lung was all above 2. 0. The tumor PET images with ^18F-FLT were clear, as well. Conclusions The uptake of ^18F-FLT in pulmonary adenocarcinoma is higher than that in normal tissues, thus the pulmonary neoplasm could be identified accurately with PET imaging. Our preliminary study of ^18F-FLT in lung carcinoma xenografts is satisfactory, and it provides a basis for further clinical study.

关 键 词：^18F-FLT 体层摄影术 发射型计算机 组织分布 肺肿瘤 模型 动物 

分 类 号：R734.2[医药卫生—肿瘤]