鸡IL-2和IL-15基因真核表达质粒对禽流感疫苗的免疫增强作用  

Immunoenhancement of eukaryotic expression plasmids with chicken IL-2 or IL-15 genes on H5 subtype avian influenza vaccine

作　　者：胡慧琼[1] 王红宁[2] 袁斌[1] 周生[1] 李晓英[1] 黄勇[1] 柳萍[1] 徐永莉[1] 

机构地区：[1]四川农业大学动物科技学院动物预防医学生物工程实验室,四川雅安625014 [2]四川大学生命科学学院,四川成都610064

出　　处：《中国兽医科学》2006年第10期805-810,共6页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2003AA241120)

摘　　要：根据GenBank中的鸡IL-2和IL-15序列设计了2对引物,采用RT-PCR法从山地乌骨鸡外周血淋巴细胞RNA中克隆了IL-2和IL-15基因,与pcDNA3.1连接构建了真核表达质粒pDNAIL-2和pDNAIL-15,研究了其对禽流感灭活疫苗的免疫增强作用。结果显示,免疫后第10d,pDNAIL-15+AI灭活疫苗组和pDNAIL-2+AI灭活疫苗组HI抗体水平显著高于AI灭活疫苗组(P<0.05)。免疫后第10 d,pDNAIL-15+AI灭活疫苗组CD4+淋巴细胞百分含量显著高于pDNAIL-2+AI灭活疫苗和AI灭活疫苗组(P<0.05);免疫后第23 d,pDNAIL-2+AI灭活疫苗组CD4+淋巴细胞百分率显著高于pDNAIL-15+AI灭活疫苗和AI灭活疫苗组(P<0.05)。证实pDNAIL-2和pDNAIL-15能提高禽流感灭活疫苗的免疫效果。Two pairs of specific primers for chicken interleukin 2 （CHIL-2） and interleukin-15 （CHile-15） genes were designed according to the published gene sequences of CHIL-2 and CHIL-15. The eukaryotic expression plasmids with CHIL-2 gene or CHIL-15 gene, named pDNAIL-2 and pDNAIL-15, were constructed by subcloning CHIL-2 gene or CHIL-15 gene into pcDNA3.1 （＋）, respectively. The immunoenhancement of pDNAIL-2 and pDNAIL-15 on H5 subtype avian influenza vaccine was observed by detection of HI antibody titers and percentages of CD4^＋ and CD8^＋. In results, on day 10 post-inoculation （PI）, the HI antibody titers in the pDNAIL-15＋AI vaccine group and the pDNAIL-2＋AI vaccine group were significantly higher than those in the AI vaccine group（P〈0.05）. The percentage of CD4^＋ in the pDNAIL-15＋AI vaccine group was significantly higher than that in the pDNAIL-2＋AI vaccine group and the AI vaccine group on day 10 PI（P〈0.05）. The percentage of CD4^＋ in the pDNAIL-2＋AI vaccine group was significantly higher than that in the pDNAIL-15＋AI vaccine group and the AI vaccine on day 23 PI（P〈0.05）. These results indicated that pDNAIL-2 and pDNAIL-15 have adjuvant effects on the AI vaccine.

关 键 词：鸡白细胞介素-2 鸡白细胞介素-15 禽流感疫苗 HI抗体 

分 类 号：S852.659.5[农业科学—基础兽医学]