声动力疗法对C6胶质瘤细胞的生物效应研究  

Biological effect of sonodynamic therapy on C6 glioma cells

作　　者：宋大勇[1] 岳武[1] 王智[1] 赵钊[1] 张旭[2] 孟祥喜[2] 王策[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经外科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院神经外科,黑龙江哈尔滨150001

出　　处：《中国微侵袭神经外科杂志》2006年第10期459-461,共3页Chinese Journal of Minimally Invasive Neurosurgery

摘　　要：目的探讨声动力化学疗法(SDT)治疗脑胶质瘤的可能性。方法采用四唑盐比色法,即MTT法测定C6胶质瘤细胞在SDT组、超声组、血啉甲醚(HMME)组、对照四组6 h的抑瘤率,流式细胞学(FCM)检测四组6 h的凋亡率,透射电镜观察四组6 h亚细胞结构的变化。结果SDT组抑瘤率为77.61%、凋亡率为38.34%,显著增高;超声组也有一定的抑瘤率、凋亡率;HMME组抑瘤率、凋亡率与对照组无明显差别。透射电镜观察对照组、HMME组C6细胞无明显变化,超声组部分细胞呈早期凋亡表现,SDT组细胞呈现凋亡或坏死状态。结论SDT能显著杀伤体外C6胶质瘤细胞,并诱导其凋亡。Objective To explore the feasibility of treating brain glioma by sonodynamic therapy （SDT）. Methods After cultivation for 6 hours, the inhibition rate, apoptosis rate and the ultrastructural changes of C6 glioma cells were assayed in SDT group, ultrasound group, HMME （hematoporphyrin monomethyl ether） group and control group by MTT, flow cytometry （FCM） and transmission electron microscopy respectively. Results The inhibition rate and apoptotic rate of C6 glioma cells were 77.61% and 38.34% respectively in SDT group, suggesting a significant increase. Ultrasound group also obtained some effects, while HMME group showed no significant differencc compared with the control group. Observed under transmission electron microscope, the changes of C6 glioma cells in control group and HMME group were not significant, and early-phase apoptosis was detected in ultrasound group, while the cells in SDT group displayed morphological features ofapoptosis or necrosis. Conclusion SDT can kill C6 glioma cells significantly and induce apoptosis of the tumor cells in ritro.

关 键 词：超声疗法 血卟啉类 神经胶质瘤 肿瘤细胞 培养的 

分 类 号：R739.41[医药卫生—肿瘤]