负载乳腺癌抗原单核细胞来源树突状细胞迁移能力的调控因素研究  

Promotion of the migratory ability of human monocyte-derived dendritic cells

作　　者：朱旬[1] 甄林林[1] 查小明[1] 郑伟[1] 王汉晋[1] 王萱仪[1] 武正炎[1] 

机构地区：[1]南京医科大学第一附属医院普外科,南京210029

出　　处：《中国免疫学杂志》2006年第10期909-913,共5页Chinese Journal of Immunology

摘　　要：目的:体外培养乳腺癌抗原负载的人单核细胞来源DC,应用不同刺激因子使其表面CCR7表达上调,在体外观察其迁移能力。方法:用rhGM-CSF和rhIL-4诱导MoDCs,并负载乳腺癌抗原后,分别与PGE2、LTC4或Bryo-1共培养,检测其表型及其功能变化。结果:PGE2、LTC4和Bryo-1在体外不影响MoDCs刺激淋巴细胞增殖能力和自体特异性淋巴细胞杀伤活性。与对照组MoDCs相比,PGE2和LTC4作用MoDCs后CD86表达增高,可以上调CCR7mRNA和蛋白表达(P<0·05)。PGE2可以使MoDCs对其配体CCL19和CCL21反应性增强(P<0·05),而LTC4仅能使MoDCs对CCL19反应性增强。Bryo-1虽然可以促进MoDCs成熟,使其CCR7mRNA表达增强,但在CCR7蛋白表达、迁移能力方面与对照组相似。结论:以PGE2和LTC作用MoDCs后可以通过上调其CCR7表达,促进其迁移趋化能力。Objective： To promote the expression of chemokine receptor CCR7 on human monoeyte-derived dendritic cells, Metbods：Monoeyte-derived dendritic cells（MoDCs） were cultured medium containing rhGM-CSF and rhlL-4. After loaded with breast carcinoma antigen,DCs were stimulated with PGE2 ,LTC4 or Bryo-1 for 1 day, Cellar surface molecules such as CD86 and CCR7 were analyzed by flow eytometry. The expressions of CCR7 on surface of MoDCs also were detected with method of RT-PCR and Western blot. The ehemotaxis assay was measured by migration through a polyearbonate filter with 5 μm pore size in transwell chambers. The competence of inducing mixed lymphocyte response （MLR） and specific eytotoxie T lymphocyte （CTL） were detected with MTT. Resuits： Compared with control group, PGE2 and LTC4 upregulated surface makers of CD86 and CCR7, but had no effect on MLR and CTL. In vitro ehemotaxis assay,MoDCs stimulated with PGE2 were more sensitive to the CCR7-1igands CCL19 and CCL21 than that of control group. But LTC4 group was only sensitive to CCL19. PGE2 and LTC4 enhanced the expression of CCR7 mRNA and protein. However Bryo-1 only enhanced the expression of CD86, and CCR7 mRNA. In vitro ehemotaxis assay, the effect of MoDCs stimulated by Bryo-1 was not better than that of control group. Conelusion：PGE2 and LTC4 can upregulate CCR7 surface expression of MoDCs, enhanced effieieney in migration toward ehemokines.

关 键 词：树突状细胞 乳腺肿瘤 趋化因子 免疫治疗 

分 类 号：R730.51[医药卫生—肿瘤]