IL-1B基因多态性和H.pylori感染与汉族人胃癌的相关性研究  

Association of IL-1B gene polymorphism and H.pylori infection with gastric cancer in Chinese patients with Han nationality

作　　者：江绍伟[1] 章晓联[2] 宁勇[1] 高尚民[1] 姚群峰[1] 

机构地区：[1]湖北中医学院医学检验与技术系,武汉430064 [2]武汉大学医学免疫学系,武汉430071

出　　处：《中国免疫学杂志》2006年第10期959-961,共3页Chinese Journal of Immunology

基　　金：湖北省教育厅青年项目资助(Q200516001)

摘　　要：目的:通过比较胃癌患者与匹配人群的幽门螺旋杆菌(Helicobacter pylori,H.pylori)感染率和IL-1B基因多态性,探讨IL-1B基因多态性是否增加H.pylori感染后胃癌发生的危险性。方法:采用聚合酶链反应-限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)分析法检测胃癌低发区84例胃癌患者和84例与之性别、年龄匹配的普通人群的lL-1B基因多态性。采用酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测上述人群中的H.pylori感染率。结果:①胃癌患者IL-1B-511T/T基因型频率显著高于性别、年龄匹配的对照人群(P<0·05),IL-1B-31T/T基因型频率在两组间无显著差异(P>0·05)。84例胃癌患者的H.pylori感染率显著高于对照人群(P<0·01)。胃癌患者H.pylori阳性感染者IL-1B-511T/T基因型个体显著多于对照人群。结论:H.pylori感染者胃癌组织中IL-1B-511T/T基因型为主,提示IL-1B-511T/T基因型可能增加H.pylori感染后中国汉族人群发生胃癌的危险性,而IL-1B-31基因型与H.pylori感染后中国汉族人群胃癌发生无显著相关性。Objective：The H. pylori infection rate of and IL-1B gene polymorphism in gastric cancer patients and normal people were investigated. The effect of IL-1B gene polymorphism on the development of gastric cancer inducing the H. pylori infection. Methods：IL-1B polymorphisms were analyzed with PCR-RFLP in 84 eases of gastric cancer and 84 eases of normal people in low-prevalence region. The tt. pylori infeotion rates were examined with ELISA. Results：T/T carrier frequencies of IL-1B-511 was remarkably higher in gastric cancer patients compared with the normal people（ P 〈 0. 05 ） ; TIT ear＇tier frequencies of IL-1B-31 was similar in all of the population. H. pyloriinfection rate of 84 eases gastric cancer patients was remarkably higher than normal people（ P 〈 0.01 ）. T/T earrier frequencies of IL-1B-511 was remarkably higher in gastric cancer patients H. py/or/infected compared with the normal people. Condusion：IL-1B-511 TIT genotype might be increase the risk of gastric cancer infected by H. pylori in Chinese population. IL-1B-31 genotype do not increases the risk of gastric cancer infected by H. pylori.

关 键 词：胃肿瘤 IL-1B 基因多态性 幽门螺旋杆菌 

分 类 号：R735.2[医药卫生—肿瘤]