蝎毒对人乳腺癌MCF-7/ADM细胞多药耐药的影响及机制研究  

Effect of BMK on multidrug resistance reversal and glutathione s-transferase activity in human breast cancer MCF-7/ADM cell line

作　　者：解霞[1] 赵瑾瑶[2] 高清波[1] 杨佩满[2] 

机构地区：[1]大连大学医学院解剖学教研室,辽宁大连116622 [2]大连医科大学组织胚胎学教研室,辽宁大连116027

出　　处：《大连医科大学学报》2006年第5期376-377,383,共3页Journal of Dalian Medical University

摘　　要：[目的]探讨蝎毒(BMK)对人乳腺癌MCF-7/ADM细胞多药耐药的逆转及谷胱甘肽S转移酶(GST)活性的影响。[方法]实验分两组:MCF-7/ADM耐药细胞组;MCF-7/ADM+蝎毒组。采用MTT法测定蝎毒的细胞毒性和抗药性逆转,流式细胞术测定蝎毒对耐药细胞凋亡百分率的影响,紫外分光光度术测定蝎毒对谷胱甘肽S-转移酶(GSTs)活性的影响。[结果]与MCF-7/ADM耐药细胞组相比:①非细胞毒性剂量(3.0μg/mL)蝎毒能显著降低MCF-7/ADM的IC50(P<0.01),逆转倍数为1.52倍。②蝎毒(3.0μg/mL)显著增强对耐药细胞的凋亡诱导作用,凋亡率由(8.9±0.01)%上升为(12.6±0.21)%(P<0.01)。③蝎毒(3.0μg/mL)显著降低耐药细胞内谷胱甘肽S转移酶的活性(P<0.01)。[结论]蝎毒能够部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,增加ADM对肿瘤细胞的凋亡诱导作用,其逆转机制与降低耐药细胞内GST酶活性有关。[Objective] To study the effect of inducing apoptosis and resistance reversing on BMK combined with ADM in human breast cancer MCF-7/ADM cell line. [Methods] The major groups were divided as follows： MCF - 7/ADM cell lines; MCF - 7/ADM cell lines with BMK. The cytotoxicity and the reversal of resistance of BMK were assessed by MTT assay. The apoptosis percentage were determined by the flow cytometry. The glutathione s - transferase activity were determined by the UV - spectrophotometry. [Results] Compared with MCF - 7/ADM cell lines： ① The non - cytotoxic dose of BMK （3 μg/mL） significantly decreased the IC50 value of ADM （P 〈 0.01）. Its reversal folds is 1.52. ② BMK （3 μg/mL） increased the apoptosis inducing effect of ADM to MCF - 7/ADM cells, which of apoptosis percentage was increased from （8.9±0.01） % to （12.6±0.21） % （P 〈 0.01）. ③ BMK （3μg/mL） could significantly decrease glutathione s - transferase activity in MCF - 7/ADM cells （P 〈 0.01）. [Conclusion] BMK could partially reverse resistance to ADM and increase the apoptosis in human breast cancer MCF - 7/ADM cell line, which is related to decreasing glutathione s - transferase activity.

关 键 词：蝎毒 逆转 谷胱甘肽S转移酶 MCF-7/ADM细胞株 

分 类 号：R737.9[医药卫生—肿瘤]