银杏外种皮粗多糖提取工艺研究  

Study on the Extraction Technology of Crude Polysaccharides from Ginkgo biloba Sarcotesta

作　　者：吴向阳[1] 茆广华[2] 仰榴青[3] 吴静波[1] 朱小宦[2] 

机构地区：[1]江苏大学化学化工学院,江苏镇江212013 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]江苏大学药学院,江苏镇江212013

出　　处：《食品科学》2006年第10期372-375,共4页Food Science

基　　金：江苏省天然药物研究与创制实验室开放课题(KF2005005);镇江市科技计划项目(NY2005037);江苏大学高级技术人才科研启动基金项目(04JDG030)

摘　　要：目的:研究银杏外种皮中粗多糖的水提最优工艺。方法:采用苯酚-硫酸法测定多糖含量,以多糖提取率为考察指标,在单因素试验结果的基础上,进行正交试验设计,考察料液比、温度、时间等因素对提取率的影响。结果:温度是影响粗多糖提取率的重要因素。水提法最优工艺条件为温度100℃、时间1h、料液比1:12、提取次数3次,此条件下多糖提取率达95.09%,粗多糖得率为23%。结论:该提取工艺的多糖提取率高,可用于银杏外种皮中粗多糖的提取。Objective： To study the optimal water extraction technology of polysaccharides from Ginkgo biloba sarcotesta.Methods： The content of the polysaccharides was determined by the method of phenol-sulphate acid. The extraction rate was regarded as an examine target. On the basis of single factor tests, orthogonal experiment design was applied to analyze the influence of factors including solid-liquid ratio, extraction temperature and time on the extraction rate of the polysaccharides. Results： Temperature is an important factor for the extraction rate of the polysaccharides. The optimal technology condition was obtained. The extraction temperature is 100℃, the time is 1 h, and the solid-liquid ratio is 1：12. The extraction rate of polysaccharides and the yield of crude polysaccharides were 95.09% and 23%, respectively. Conclusion： The optimal process is applicable to the extraction of crude polysaccharides from Ginkgo biloba sarcotesta.

关 键 词：银杏外种皮 多糖 提取工艺 

分 类 号：R284.2[医药卫生—中药学]