己烯雌酚残留的三种检测方法比较研究  

Comparison Study on Three Methods to Assay DES Residue with HPLC,FS and CZE

作　　者：陈眷华[1] 王文博[2] 徐在品[1] 毛以智[3] 卢占军[1] 

机构地区：[1]贵州大学动物科学学院动物医学系,贵州贵阳550025 [2]中国海洋大学生命科学与技术学部,山东青岛400044 [3]贵州省遵义市畜牧局,贵州遵义563000

出　　处：《食品科学》2006年第9期214-218,共5页Food Science

基　　金：国家自然科学基金资助项目(10572029)

摘　　要：己烯雌酚(diethylstilbestrol,DES)是一种在畜牧业生产中广泛应用的人工合成的非甾体类雌激素。在己烯雌酚长期生产实践中研究发现,己烯雌酚具有以生殖毒性为主的严重毒副作用。本实验就高效液相色谱法(highperformanceliquidchromatography,HPLC)的、荧光分光光度计法(fluorescencespectrophotometer,FS)、毛细管电泳法(capillaryzoneelectrophoresis,CZE)三种检测方法作比较。旨在寻找一种快速、高效、可行的动物组织中己烯雌酚残留检测方法。实验表明高效液相色谱法最小检出量为6.25×10-3mg/ml,荧光光度法的最小检出量8.04×10-3mg/ml,毛细管区带电电泳法的检测所用时间为4min,具有较快的检测速度,仪器便宜、方法简便、准确、试剂用量小,而且废液容易处理、污染小。可见CZE法在基层实际检测中是一种有效、可行的检测方法。Diethylstilbestrol is artificially produced and widely used estrogenic hormone in stockbreeding production. In the long period of using DES, the seriousness of toxic hazard to the genital system has been discovered. In this experiment the three methods of HPLC, FS, CZE were compared in order to find a highly sensitive, quick and feasible method especially suitable for detecting residue quantity of DES in animal tissues. The results showed that the minimum detectable quantity of HPLC and FS was 6.25 ×10^-2 and 8.04 ×10^-2 mg/ml respectively. The CZE was characterized by being quick, economic, simple, accurate with, little quantity of reagent required and small liquid waste disposed, so only slightly polluted. Hence accding to the actual national conditions, the CZE is an effective and feasible method applicable in grass roots.

关 键 词：己烯雌酚 高效液相色谱 荧光分光光度计 毛细管区带电电泳 

分 类 号：S852.44[农业科学—基础兽医学]