蜂王浆冻干粉对HepG2细胞葡萄糖消耗作用及对糖尿病小鼠降糖作用的实验研究  

Lyophiled Royal Jelly In vitro and In vivo Hypoglycemic Studies on Effects of Glucose Consumption in Cultured HepG2 Cells and Blood Sugar in Alloxan-induced Diabetic Mice

作　　者：汪宁[1] 朱荃[2] 周义维[1] 童黄锦[1] 

机构地区：[1]安徽中医学院药学院,安徽合肥230031 [2]南京中医药大学国家规范化中药药理实验室,江苏南京210029

出　　处：《食品科学》2006年第9期233-235,共3页Food Science

基　　金：安徽省高等学校青年教师科研资助计划项目(2005jq1099)

摘　　要：目的:研究蜂王浆冻干粉体内外降糖作用。方法:采用与人肝细胞表型相似的HepG2细胞,检测24h后培养液中葡萄糖的消耗量,用MTT法监测细胞增殖的情况;以四氧嘧啶造成小鼠糖尿病模型,观察蜂王浆对糖尿病模型小鼠的降糖作用。结论:蜂王浆冻干粉可使高糖状态下HepG2细胞的葡萄糖消耗量增加,对胰岛素刺激的HepG2细胞的葡萄糖消耗量增加有协同作用;能降低四氧嘧啶所致糖尿病小鼠的血糖。Objective： To observe the hypoglycemic effects of royal jelly in vitro and in vivo. Methods： HepG2 cells, the phenotype of which is similar to that of the human hepatocytes, were used to assay the 24h, consumption of glucose in cultured fluid. MTT assay was used to monitor the proliferation of the HepG2 cells. The experimental diabetic mice induced by aUoxan well established and determined with the changes of blood sugar in diabetic mice. Conclusions： Royal jelly could increase the glucose consumption of HepG2 cells and enhance the glucose consumption in synergism with insulin. Lyophiled royal jelly could also decrease significantly the blood sugar in alloxan-induced diabetic mice.

关 键 词：蜂王浆 HEPG2细胞 糖尿病 四氧嘧啶 葡萄糖消耗 血糖 

分 类 号：R332[医药卫生—人体生理学]