用TK基因突变试验评价丙烯酰胺的致突变性  

Application of tk Mutation Assay for Mutagenicity with Acrylamide

作　　者：郑卫平[1] 吕敬章[1] 孙秀发[2] 杨雪锋[2] 庞红[2] 

机构地区：[1]深圳出入境检验检疫局,广东深圳518067 [2]华中科技大学同济医学院营养与食品卫生学系,湖北武汉430030

出　　处：《食品科学》2006年第9期250-252,共3页Food Science

摘　　要：用小鼠淋巴瘤细胞TK基因突变试验对丙烯酰胺的致突变性进行了研究。实验设0.10、0.25、0.50、0.75mg/ml4个剂量组和阴性、阳性对照组。结果发现,随着丙烯酰胺剂量的增加,细胞相对悬浮增长率下降,tk位点的突变频率显著增加,0.5、0.75mg/ml两剂量组的突变频率与阴性对照组相比有显著性差异,并且有剂量效应关系,表明丙烯酰胺有明显的细胞毒性,并可诱发tk位点的突变。The mutagenic cell-L5178Y-3.7.2C cell-was treated with different concentrations of acrylamide （0.10, 0.25, 0.50 and 0.75mg/ml respectively）. The results showed that with the increasing of the concentration of acrylamide, Relative Suspension Growth （RSG） decreases, and tk locus mutation frequency increases. Moreover, tk locus mutation frequency induced by 0.5, 0.75mg/ml of acrylamide is dosage-dependently higher than spontaneous mutation frequency. The results suggested that the acrylamide induces mutagenicity at higher concentration.

关 键 词：丙烯酰胺 致突变性 TK基因突变 

分 类 号：R992[医药卫生—毒理学]