菱灵颗粒有效成分的抗肿瘤作用研究  

Anti-cancer Effects of Ling Ling Pellet

作　　者：牛凤兰[1] 李晨旭[1] 王学东[2] 程舸[1] 梁睿[1] 董卿[1] 王天行 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]长春市朝阳区疾病预防控制中心,吉林长春130021 [3]长春市朝阳区卫生局,吉林长春130021

出　　处：《食品科学》2006年第9期253-256,共4页Food Science

基　　金：吉林省发改委基金项目(吉发改高技联字[2004]987号)

摘　　要：目的:研究菱灵颗粒有效成分—化合物Ⅰ在体外对Hela细胞的作用及其作用机制。方法:采用四甲基偶氮唑盐(MTT)还原法检测化合物Ⅰ对肿瘤生长抑制作用,应用光学显微镜、透射电子显微镜观察细胞形态,流式细胞仪检测细胞周期及细胞凋亡情况。结果:化合物Ⅰ对人宫颈癌细胞生长具有明显的抑制作用及诱导细胞凋亡作用,且这种作用有剂量依赖关系,化合物Ⅰ25、12.5、6.25mg/L剂量组30h抑瘤率为52.04%、34.44%、23.72%,100、50、25、12.5、6.25、3.125mg/L剂量组30h抑瘤率显著正相关,相关系数r=0.9860(p<0.01),IC50值为10.9mg/L。透射电子显微镜对化合物Ⅰ25、12.5、6.25mg/L剂量组受试细胞观察,均出现不同程度细胞凋亡现象,细胞核明显皱缩、染色质趋边凝聚、线粒体空化等特征。流式细胞仪检测发现凋亡峰。结论:化合物Ⅰ对Hela细胞增殖抑制作用明显并且有诱导细胞凋亡作用。Objective ： To investigate the effects of Compound Ⅰ abstracted from ling ling pellet on human Hela cell so as to explore its mechanism. Methods： Growth inhibition was assayed with MTr. The morphological changes of Hela cells induced by compound Ⅰ were observed with transmission electronic microscope and phase contrast microscope. Cell division cycle distribution and apoptosis affected by compound Ⅰ was determined with flow cytometry. Results： The growth of human Hela cells is significantly inhibited which display strong in a dose and time-dependant manners against and induced apoptosis of Hela cells. Hela cell with estimated IC50 values of 10.9mg/L, concentration of 25, 12.5, 6.25mg/L inhabiting rate is 52.04%, 34.44%, 23.72% after 30h of treatment with compound I. Concentration of 100, 50, 25, 12.5, 6.25, 3.125mg/L inhabiting rate is good correlation （r=0.9860, p 〈 0.01）. Expose of Hela cells to 25, 12.5, 6.25mg/L of compound Ⅰ induced nuclear shrinkage, chromatin condensation and margination against nuclear envelope as seen in apoptotic cells. The follow cytometry profile revealed that treatment with different Concentrations compound Ⅰ inducted apoptosis. Conclusion： Growth of human Hela cells was significantly inhibitory and apoptosis may play an important role in the anti-tumor effect of active compound Ⅰ.

关 键 词：菱灵颗粒 HELA细胞 细胞凋亡 

分 类 号：R967[医药卫生—药理学]