大鼠C6脑胶质瘤模型的建立  

Establishment of Cell Line C6 Brain Glioma Models in Rats

作　　者：冯军[1] 赵洪洋[1] 胡雪芝[2] 石新东[1] 蒲建章[1] 靳峰[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经外科,湖北武汉430022 [2]济宁市第一人民医院,山东济宁272000

出　　处：《中国临床神经外科杂志》2006年第10期613-615,共3页Chinese Journal of Clinical Neurosurgery

摘　　要：目的通过不同方法建立大鼠脑胶质瘤模型,观察其生长特性,比较各种方法的优劣。方法体外培养大鼠C6胶质瘤细胞,分别取浓度为1.0×105个/10μl、1.0×106个/10μl、1.0×107个/10μl细胞悬液,立体定向接种于Wistar大鼠脑右侧尾状核区,观察不同接种量实验鼠的生存状态、成瘤情况及脏器转移灶,并采用免疫组织化学方法检测其胶质纤维酸性蛋白(GFAP)、血管内皮生长因子(VEGF)的表达。结果各种接种量的实验组成瘤率均为100%,未见颅外转移病灶,在组织病理学上接近人脑胶质瘤,瘤细胞病理性核分裂像多见,GFAP蛋白呈散在阳性表达,VEGF蛋白呈强阳性表达,瘤组织内有出血和坏死以及丰富的微血管。实验周期短,重复性高。结论C6细胞接种Wistar大鼠脑胶质瘤动物模型,肿瘤成瘤率高,颅内生长稳定,肿瘤组织病理学特性与人脑胶质瘤相似,可作为临床胶质瘤基础研究的理想模型。实验范围内部分时段不同接种剂量肿瘤生长速度有显著性差异,可根据病因学、实验治疗或药效学等不同研究需要选择不同接种量。Objective To establish brain glioma models of C6 cells with different methods in rats and to observe the characteristics of the tumors growth. Methods Rat C6 glioma cells were cultured in vitro. Ten μl of cell suspensions containing 1.0×10^5 C6 eells/10μl, 1.0×10^6 C6 cells/10μl and 1.0×10^7 C6 cells/10μl was stereotaxically inoculated into the right caudate nucleus of the brain in the rats of different experimental groups, respectively. The moving and survival states of rats with gliomas were observed. The examinations of the tumors formation, volumes, metastasis and bistopatbology were performed. The expression of glial fibrillary acidic protein（GFAP）, and vascular endothelial growth factor（VEGF） in the tumors were determined by immuobistocbemistry. Results All the rats receiving inoculation of different dose of C6 cells developed brain tumors without extracranial metastasis. The rat C6 gliomas resembled histopathologically human glioma. The tumors contained many cells with remarkable cytoplasmic and nuclear polymorphism, and pathological mitosis figures. The scattered positive expression of VEGF was observed in tumor cells. There was the strongly positive expression of GFAP in the tumors. Conclusions The successful rate of C6 brain tumor model established by the intracerebral inoculation of C6 cells is very high in rats. The tumor established in the experiment is similar to human glioma in histopathological features and it can be used as a good model to study the glioma. The tumors model established by inoculation of different dose of C6 cells can be selected according to different needs of different experiments because their growth speeds are different from each other.

关 键 词：胶质瘤 动物模型 接种量 

分 类 号：R730.264[医药卫生—肿瘤] R73-35[医药卫生—临床医学]