Expression of aquaporin-1 in SMMC-7221 liver carcinoma cells promotes cell migration  

作　　者：LI Yongming FENG Xuechao YANG Hong MA Tonghui 

机构地区：[1]Membrane Channel Research Laboratory, Northeast Normal University,Changchun 130024, China

出　　处：《Chinese Science Bulletin》2006年第20期2466-2471,共6页

基　　金：the National Natural Science Fund for Distinguished Young Scholars （Grant No. 30325011）; National Natural Science Foundation of China （Grant No. 30470405）; Distinguished Young Scholars Fund of Jilin Province （Grant No. 20030112）; Excellent Young Teachers Program of Ministry of Education of China.

摘　　要：Migration of tumor cells is a crucial step in tumor invasion and metastasis. Here we provide evidence that aquaporin expression is involved in tumor cell migration. RT-PCR, immunofluorescence and Western blot analysis demonstrated the AQP1 protein expression on the plasma membrane of SMMC-7221 human hepatoma cells. SMMC-7221 cell clones with high (SMMC-7221hPf) and low (SMMC-7221lPf) water permeability were identified by functional assays with corresponding high and low AQP1 expression. Cell migration rate was remarka- bly higher in SMMC-7221hPf cells than SMMC-7221l Pf cells, assessed by Boyden chamber and wound healing assays, whereas cell growth and adhesion were not different. Adenovirus-mediated AQP1 ex- pression in SMMC-7221lPf cells increased their water permeability and migration rate. These results pro- vide the first evidence that aquaporin-mediated membrane water permeability enhances tumor cell migration and may be associated with tumor invasion and metastasis.Migration of tumor cells is a crucial step in tumor invasion and metastasis. Here we provide evidence that aquaporin expression is involved in tumor cell migration. RT-PCR, immunofluorescence and Western blot analysis demonstrated the AQP1 protein expression on the plasma membrane of SMMC-7221 human hepatoma cells. SMMC-7221 cell clones with high （SMMC-7221hPf） and low （SMMC-7221/Pf） water permeability were identified by functional assays with corresponding high and low AQP1 expression. Cell migration rate was remarka- bly higher in SMMC-7221 hPf cells than SMMC-7221/ Pf cells, assessed by Boyden chamber and wound healing assays, whereas cell growth and adhesion were not different. Adenovirus-mediated AQP1 expression in SMMC-7221/Pf cells increased their water permeability and migration rate. These results provide the first evidence that aquaporin-mediated membrane water permeability enhances tumor cell migration and may be associated with tumor invasion and metastasis.

关 键 词：细胞迁移 瘤转移 细胞容积 肝肿瘤 SMMC-7221 

分 类 号：R735.7[医药卫生—肿瘤]