棉花赤霉素氧化酶同源基因GhGA20ox1在烟草中的功能表达(英文)  

Functional Expression of the Cotton Gibberellic Acid Oxidase Homologous Gene GhGA20ox1 in Tobacco

作　　者：肖月华[1] 叶应福[1] 冯怡[1] 李先碧[1] 罗明[1] 侯磊[1] 罗小英[1] 李德谋[1] 裴炎[1] 

机构地区：[1]西南大学生物技术中心,农业部生物技术和作物品质改良重点实验室,重庆400716

出　　处：《植物生理与分子生物学学报》2006年第5期563-569,共7页Journal Of Plant Physiology and Molecular Biology

基　　金：国家重点基础研究发展规划资助项目(973)(No.2004CB117300);国家自然科学基金项目(Nos.30270798,30530490)资助~~

摘　　要：为研究棉花GA20-氧化酶同源基因GhGA20ox1的功能,将该基因转入本明烟(N.benthamiana)中进行超量表达。RT-PCR分析表明GhGA20ox1基因在转基因植株中得到了不同水平的表达。GhGA20ox1基因的超量表达促进了本明烟中的GA4+7合成,并导致赤霉素过量的表型出现。转基因本明烟的表型变化程度与GhGA20ox1基因的表达水平和GA4+7的含量一致。这些结果表明,GhGA20ox1基因编码一个有功能的GA20-氧化酶,能够在转基因烟草中促进活性GA(GA4+7)的合成。To determine the physiological function of GhGA20ox1, a homologous gene of GA 20-oxidase from elongating cotton fibers, we expressed this gene ectopically in Nicotiana benthamiana. Reverse transcription-PCR analysis showed that the GhGA20ox 1 gene was expressed in the transgenic plants at various levels. It was demonstrated that overexpression of GhGA20ox1 enhanced preferentially the GA4＋7 biosynthesis in N. benthamiana and conferred GA-overproduction characters to transformants. The extent of phenotypic alteration in the transgenic plants was found to correlate with the transcriptional levels of GhGA20ox1 and GA contents. Results indicated that the GhGA20ox1 gene promoted the biosynthesis of the active GAs （GA4＋7） in transgenic tobacco plants therefore represents a useful gene for manipulating GA levels.

关 键 词：赤霉素 GA 20-氧化酶 棉花GA 20-氧化酶基因 GA合成 基因工程 

分 类 号：S562[农业科学—作物学]