The approaches in detecting cell cycle specificity of Fas-mediated apoptosis in leukemia cell lines and activated PBLs in vitro  

作　　者：Xiaojun He Jing Hu Xiaolan Li Hui Xiao Deding Tao Haocheng Long Jianping Gong 

机构地区：[1]Tongji Cancer Research Institute/Molecular Medical Center, Tongji Hopital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2006年第5期379-382,共4页中德临床肿瘤学杂志（英文版）

基　　金：Supported by the Major State Basic Research Development Program of China (973 program) (No. 2004CB518705, 2002CB513100-2) and Clinical Key Subject Foundation from Ministry of Health of China "Cell Cycle Diag-nosis and Analysis in Clinical Tumor (III)".

摘　　要：Objective： To establish a system in detecting the cell cycle specificity induced by recombinant human Fas ligand in vitro, so as to provide a reliable platform for further exploring the mechanism of cell cycle control and regulation in Fas-mediated apoptosis. Methods： The target cells-leukaemia cell lines and activated peripheral blood lymphocytes stimulated by phytohemagglutinin were incubated with recombinant human Fas ligand for 6 to 36 h, apoptosis was detected by sub-G1, common annexin-Ⅴ/PI and modified annexin Ⅴ and propidium iodide （API） methods and analysed by flow cytometry. Results： The modified API method demonstrated that Fas-mediated apoptosis was cell cycle specific and initiated at G1 phase. The common annexinⅤ/PI method showed the most appropriate condition for the detection of typical cell cycle-specific apoptosis. The sub-G1 method could only illuminate late apoptosis and DNA histogram. Conclusion： Fas-mediated apoptosis was cell cycle-specific and initiated at G 1 phase. Based on the modified API and common AnnexinⅤ/PI methods, the establishment of stable and typical cell cycle-specific model in Fas-mediated apoptosis in vitro was feasible.

关 键 词：Fas-mediated apoptosis cell cycle flow cytometry leukaemia cell peripheral blood lymphocyte 

分 类 号：R733.7[医药卫生—肿瘤]