蛋白脂蛋白在两型星形胶质细胞和少突胶质细胞中的表达  

Expression of proteolipid protein in type-1, type-2 astrocytes and oligodendrocytes

作　　者：张晔[1] 李春鹏[1] 夏春林[1] 沈慧[1] 孙文阁[1] 

机构地区：[1]苏州大学医学院解剖学教研室暨细胞神经生物学研究室,苏州215123

出　　处：《解剖学杂志》2006年第5期536-538,580,F0002,共5页Chinese Journal of Anatomy

基　　金：江苏省高校自然科学研究计划(05KJB310121);江苏省分子神经生物学重点实验室项目(KJS02050)

摘　　要：目的:探讨蛋白脂蛋白(PLP)在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达。方法:用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。结果:在O-2A谱系来源的成熟2型星形胶质细胞和少突胶质细胞中PLP抗体标记为阳性,而在T1A谱系来源的成熟1型星形胶质细胞中则未检测到PLP的表达,从而在蛋白质水平验证本室研究两型星形胶质细胞基因表达谱差异基因芯片结果中PLP mRNA在T2A中高表达,而在T1A中低表达的现象。结论:PLP等脂代谢相关基因可能在O-2A谱系发生和分化过程中起重要作用,O-2A谱系与神经髓鞘发生以及脑内脂质代谢内在机制密切相关。Objective： To explore the expression of proteolipid protein （PLP） in mature type-2 astrocytes, type-2 astrocytes and oligodendroeytes. Methods： Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes. Results： PLP expressed in type-2 astrocytes and oligodendrocytes from O-2A lineage, but not in type-1 astrocytes from T1A lineage. Accordingly, we verified that PLP mRNA was strongly expressed in T2A and weakly expressed in T1A. Conclusion： Lipid metabolism related genes, such as PLP, play important roles in the generation and differentiation of O-2A lineage, which is closely correlated with myelogenesis and internal mechanism of lipid metabolism in the brain.

关 键 词：星形胶质细胞 少突胶质细胞 蛋白脂蛋白 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]