戊四氮对神经元样PC12细胞内钙离子浓度和核因子κB活化的影响  

Effects of pentylenetetrazol on intracellular Ca ^(2+) concentration and the activation of NF-κB in neuron-like PC12 cells

作　　者：李卫华[1] 朱遂强[1] 唐荣华[1] 笱玉兰[1] 王开颜[1] 阮旭中[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《临床神经病学杂志》2006年第5期349-351,共3页Journal of Clinical Neurology

基　　金：国家自然科学基金资助项目(30170333)

摘　　要：目的观察戊四氮(PTZ)对神经元样PC12细胞内钙离子(Ca2+)浓度和核因子κB(NF-κB)活化的影响,进一步探讨癫的发病机制。方法将传代培养的神经元样PC12细胞随机分为PTZ干预的PTZ组和不经PTZ干预的对照组,运用免疫荧光法检测神经元样PC12细胞内的Ca2+浓度和NF-κB核区与胞浆区的比值(FN/FC)。结果PTZ组PC12细胞NF-κBFN/FC为0.8821±0.0077,明显高于对照组的0.2161±0.0267(P<0.05),提示NF-κB活化;PTZ组PC12细胞内Ca2+浓度的荧光值(213.45±6.44)明显高于对照组的(80.73±1.01)(P<0.05)。结论PTZ能够使神经元样PC12细胞内Ca2+浓度升高和NF-κB激活。NF-κB激活可能是癫疒间形成的机制之一。Objective To investigate the epileptogenesis by observing effects of pentylenetetrazol （PTZ） on intracellular Ca2^＋ concentration and the activation of nuclear factor κappa B （NF-κB） in neuron-like PC12 cells. Methods The subcuhured neuron-like PC12 cells were divided randomly into PTZ group treated with PTZ and control group treated without PTZ. The changes of the concentration of intracellular Ca2^＋ and the expression ratio of NF-κB in nucleus and in cytoplasm （FN/FC） were measured by immunofluorescence assay. Results The relative value of FN/FC of NF-κB in PTZ group （0.8821 ±0. 0077 ） was higher than that in control group （0.2161 ±0. 0267） in neuron-like PC12 cells （ P 〈 0. 05 ）. The concentration of intracellular Ca2^＋ in PTZ group （ 213.45 ±6. 44） was higher than that in control group （ 80.73 ± 1.01, P 〈 0. 05 ）. Conclusions PTZ can increases the concentration of intracellular Ca2^＋ and activate NF-κB in neuron-like PC12 cells. The activation of NF-κB may play an important role in epileptogeneses.

关 键 词：戊四氮 核因子ΚB 神经元样PCI2细胞 钙离子 

分 类 号：R742.1[医药卫生—神经病学与精神病学]