实验性自身免疫性脑脊髓炎大鼠血CD4^+CD25^+T细胞的研究  

Study on the blood CD4^+CD25^+T cells of rats with experimental autoimmune encephalomyelitis

作　　者：张为[1] 曾丽[1] 李家鑫[1] 陆婉杏[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,南宁530021

出　　处：《临床神经病学杂志》2006年第5期365-367,共3页Journal of Clinical Neurology

基　　金：广西自然基金资助项目(桂科自0542088)

摘　　要：目的探讨实验性自身免疫性脑脊髓炎(EAE)动物模型血CD4+CD25+T细胞的变化及其意义。方法以豚鼠全脊髓匀浆(GPSCH)为抗原免疫Wistar大鼠,建立EAE的动物模型,采用三色流式细胞仪检测EAE和正常大鼠外周血CD4+CD25+T细胞的细胞数并进行比较;通过观察大鼠行为学及脑和脊髓的病理改变确定EAE。结果EAE模型大鼠的成功率为48.9%,EAE大鼠外周血CD4+CD25+T淋巴细胞数(5.29±4.00)显著低于正常对照组(12.61±2.24)(P<0.01)。结论EAE大鼠血CD4+CD25+T细胞数明显减少,CD4+CD25+T淋巴细胞对神经系统脱髓鞘疾病是一种保护因子。Objective To study the number changes of blood CD4^＋ CD25^＋T cells and its significance in rats with experimental autoimmune encephalomyelitis （EAE）. Methods Wistar rats were induced by guinea pig spinal cord homogenate （GPSCH） to establish EAE models. The number of peripheral blood CD4 ^＋ CD25 ^＋ T cells in EAE was detected by flow eytometry and compared with normal rats. The behavior and the pathological changes of brain and spine cord of rats were observed to ascertain EAE model successful. Results The achievement ratio of EAE model was 48.9%. The number of peripheral blood CD4 ^＋CD25 ^＋ T cells of EAE rats（ 5.29 ± 4. 00） was significantly lower than that in the control group（ 12.61 ±2. 24, P 〈 0. 01 ）. Conclusions The number of blood CD4 ^＋CD25 ^＋ T cells decreases significantly in the EAE rats. CD4 ^＋CD25 ^＋ T cell is a protective factor for the demyelinating disease of nervous system.

关 键 词：实验性自身免疫性脑脊髓炎 CD4 ^+CD25^+T细胞 

分 类 号：R744.5[医药卫生—神经病学与精神病学]