激动素对肝纤维化大鼠肝脏胶原变化的作用研究  

Effect of Kinetin on the Alteration of Collagens in Rats With Hepatic Fibrosis

作　　者：周健[1] 田德英[1] 张振纲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2006年第5期235-237,共3页Journal of Critical Care In Internal Medicine

摘　　要：目的:观察激动素(kinetin)对肝纤维化大鼠肝脏Ⅰ、Ⅲ型胶原含量的作用。方法:将实验大鼠随机分为3组,模型组用CCl4诱导形成肝纤维化模型;激动素组造模同时给予0.1%激动素溶液5ml·kg-1·d-1;对照组仅给予生理盐水皮下注射,均治疗12周。应用苦味酸天狼猩红偏振光法和图像分析技术对3组中的胶原含量及分布特点进行观察。结果:激动素组Ⅰ型胶原与Ⅲ型胶原含量分别为(2.107±0.833)%和(1.114±0.413)%,分别较模型组(4.473±2.311)%和(1.964±0.854)%显著降低(P<0.01)。结论:激动素能使肝纤维化大鼠肝脏Ⅰ、Ⅲ型胶原含量显著降低。Objective： To detect the effect of kinetin on type I and III collagen fibrils in rats with hepatic fibrosis. Methods： Rats were randomly divided into 3 groups： model group with CCIA induced hepatic fibrosis; Kinetin group were given 0. 1 % kinetin 0. 5 ml/100 g per day at the time of making model; control group were given normal saline subcutaneously. All the groups were treated for 12 weeks. The content and distribution of collagen fibrils were observed with both picric-sirius red polarimetry and image analysis. Results： The content of type Ⅰ and Ⅲ collagen fibrils were （2. 107 ± 0. 833） % and （ 1.114 ± 0. 413）% respectively in the kinetin group, significantly lower than those in the model group （4. 473 ± 2. 311） % and （1. 964 ± 0. 854） % respectively （P〈0. 01）. Conclusion： Kinetin may significantly decrease the content of type Ⅰ and Ⅲ collagen fibrils in rats with hepatic fibrosis.

关 键 词：苦味酸天狼猩红偏振光法激动素胶原 

分 类 号：R575[医药卫生—消化系统]