过钒酸钠对电离辐射后BET-2细胞周期阻滞及细胞凋亡的影响  

Effects of pervanadate on radiation-induced G2/M arrest and apoptosis in BET-2 cells

作　　者：杨龙[1] 许祥裕[1] 从玉文[2] 陈家佩[2] 

机构地区：[1]南京军区军事医学研究所博士后科研工作站,江苏南京210002 [2]军事医学科学院放射医学研究所

出　　处：《肿瘤研究与临床》2006年第10期655-657,共3页Cancer Research and Clinic

基　　金：国家自然科学基金资助(39800032)

摘　　要：目的观察酪氨酸磷酸酶(PTP)特异性抑制剂过钒酸钠(Per)对电离辐射诱导造血细胞周期阻滞及细胞凋亡的影响。方法BET-2细胞经1,3,5,7,10Gy60COγ射线照射,用流式细胞仪观察Per对电离辐射后细胞周期改变以及细胞凋亡的影响。结果电离辐射后,BET-2细胞出现G2/M期阻滞以及细胞凋亡,具有一定的时间效应和剂量效应;而加入Per可进一步增加G2/M期阻滞,显著减少细胞凋亡。结论过钒酸钠可进一步增加辐射诱导的细胞周期阻滞,减少细胞凋亡,为寻找治疗辐射损伤药物提供新的途径。Objective To study the effects of Pervanadate（Per） on BET-2 cells cell cycle arrest and apoptosis induced by ionizing radiation. Methods The BET-2 cells were radiated with 0, 1, 3, 5, 7, 10 Gy and divided into PTP（protein tyrosine phosphatases）-speeifie inhibitor Per-treated group and non-pervanadate group（served as a control） and then incubated after γ-ray irradiation. Cell cycle and apoptosis were measured by FCM and Hematoxylin-Eosin staining at different time phases. Results G2/M phase arrest and apoptosis were induced in a pattern of dose-effect and time-course after irradiation in the BET-2 cells. In Per-treated group, G2/M phase arrest increased more notably, and apoptosis decreased markedly. Conclusions These studies suggested that Pervanadate potentialy enhance the ratio and prolong the term of G2/M phase arrest, and facilitate the damaged hematopoietie cells to be repaired, remarkably prevent radiated-cells from apoptosis.

关 键 词：过钒酸钠 电离辐射 BET-2细胞周期 细胞凋亡 

分 类 号：R363[医药卫生—病理学]