转化生长因子-β受体Ⅰ型及Ⅱ型在大鼠牙髓炎中的表达  

An Experimental Study on the Expression of TGF-βRⅠ,Ⅱ in Rat Pulpitis

作　　者：张玲[1] 兰卫东[2] 

机构地区：[1]解放军八一医院口腔科 [2]南京大学附属口腔医院口腔内科,南京210000

出　　处：《昆明医学院学报》2006年第5期68-70,80,I0006,共5页Journal of Kunming Medical College

摘　　要：目的研究转化生长因子-β受体Ⅰ型及Ⅱ型(TGF-βRⅠ、Ⅱ)在大鼠牙髓炎模型中在不同时间动态表达及定位情况;观察转化生长因子-β受体Ⅰ型及Ⅱ型在牙髓炎中表达特点,推测它在牙髓炎发生、发展中的意义.方法通过对内毒素(LPS)诱导的大鼠磨牙实验性牙髓炎组织连续进行切片,同时超敏免疫组化分析.结果1 d组、3 d组,TGF-βRⅠ、Ⅱ在成纤维细胞中均呈弱阳性表达,成牙本质细胞呈强阳性表达,至2周修复期为阳性表达.2周以后,成牙本质细胞,前期牙本质细胞呈阳性表达减弱.3周、4周以后,成牙本质细胞、牙髓成纤维细胞、修复性牙本质呈弱阳性表达.5周以后,牙髓组织变性、坏死,呈阴性染色.结论TGF-βRⅠ、Ⅱ在大鼠牙髓炎的损伤修复过程中具有重要作用.Objective To study the dynamic expression and location of TGF -βRⅠ , Ⅱ in rat pulpitis model at different time ; To evaluate the role of TGF -βRⅠ , Ⅱ on pulp injury and reparation in rat endotoxic pulpitis. Methods To establish Wistar rat＇ s endotoxic pupitis model by application of LPS. The slides were made and examined immunohistochemically. To observe the dynamic expression and location of TGF -βRⅠ , Ⅱ. TGF-βRⅠ , Ⅱ were detected with a mouse TGF-βRⅠ , Ⅱ monoclonal antibody . Results TGF -βRⅠ , Ⅱ was weak positive in the pulp fibroblasts. Odontoblast was strongly positive. In 2 weeks, there was positive in odontoblast. Up to 3 weeks and 4 weeks, there was weak positive in odontoblast, fibroblast. Conclusion TGF-βRⅠ , Ⅱ could play an important role in pulpitis of reparative dentin forming.

关 键 词：转化生长因子-Β 受体 牙髓炎 成牙本质细胞 修复性牙本质 大鼠 

分 类 号：R781.31[医药卫生—口腔医学]