雌二醇对大鼠肝星状细胞TGF-β_1、CTGF表达的影响  

The Effect of Estradiol on the Expression of TGF- β1、CTGF in rat Hepatic Stellate Cells

作　　者：黄柳云[1] 谢建萍[1] 周建亮[1] 周定辉[1] 

机构地区：[1]中南大学湘雅医院感染病科,湖南长沙410008

出　　处：《国际医药卫生导报》2006年第19期9-13,共5页International Medicine and Health Guidance News

摘　　要：目的探讨雌二醇对肝星状细胞(HSC)的影响及其可能的作用途径。方法培养大鼠HSC,初次传代后,HSC随机分为4组,分别加入不同浓度的雌二醇,加药后72小时,噻唑蓝(MTT)法检测雌二醇对HSC增殖的影响;逆转录-聚合酶链式反应(RT-PCR)分析其对HSC的转化生长因子-β1(TGF-β1)、结缔组织生长因子(CTGF)的mRNA表达的影响;免疫细胞化学分析其对HSC的α-SMA蛋白的表达的影响。结果MTT法发现雌二醇抑制HSC的增殖;RT-PCR分析雌二醇干预组HSCTGF-β1mRNA、CTGFmRNA的表达明显下降;免疫细胞化学分析雌二醇干预组HSCα-SMA蛋白表达明显下降;并且都随浓度增加抑制作用增强,差异有显著性(P<0.05)。结论雌二醇具有抑制肝纤维化作用是因为:①可以抑制HSC的增殖、活化;②抑制了致纤维化生长因子TGF-β1及其下游信号CTGFmRNA表达。Objective To analyze the effect of estradiol on the function of hepatic stellate cells.Methods First the cultured hepatic stellate cells were randomly divided into 4 groupes and different concentrations of estradiol were added to the cell groupes ,after 72 hours incubation, then their effect on the proliferation and the expression of TGF-β1 and CTGF mRNA, α-SMA protein of HSCs were detected by MTT assay, RT-PCR and Immunocytochemistry, respectlvely. Results The effect of estradiol on the proliferation and actiation of activated HSCs was confirmed; We also found that the effect of estradiol down-regulated the expression of TGF-β1 and CTGF mRNA in mRNA level detected by RT-PCR; There were in the dose-dependent manner, the difference was significant （P〈0.05）. Conclusion Estradiol has anti-fibrosis function. This maybe results from inhibiting the following factors：①they inhibit the proliferation and actiation of activated HSCs;②they suppresse the expression of pro-fibrogenic cytokine TGF-β and is＇ downstream signaling CTGF, which play an essential role in the process of hepatic fibrosis.

关 键 词：雌二醇 肝星状细胞 转化生长因子-Β1 结缔组织生长因子 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]