β-环糊精修饰区带毛细管电泳法快速测定染料木甙、染料木素、山柰酚和芦丁  

RAPID DETERMINATION OF GENISTIN,GENISTEIN,KAEMPFEROL AND RUTIN BY CAPILLARY ZONE ELECTROPHORESIS MODIFIED BY β-CYCLODEXTRIN

作　　者：吕元琦[1] 华玉山[1] 王月伶[2] 袁倬斌[2] 

机构地区：[1]德州学院分析测试中心,德州253023 [2]中国科学院研究生院应用化学研究所,北京100049

出　　处：《理化检验（化学分册）》2006年第10期800-802,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(20175025;29875027;20235010)资助项目

摘　　要：通过研究缓冲溶液的pH和浓度、β-环糊精浓度、十二烷基硫酸钠浓度和毛细管温度等条件，建立了一种β-环糊精修饰区带毛细管电泳法快速测定染料木甙、染料木素、山柰酚和芦丁的方法。在优化的条件下，4种物质在6min内得到良好分离。染料木甙、染料木素、山柰酚和芦丁峰面积和质量浓度分别在2-400，2-300，2-200和5-500mg·L^-1范围内呈线性关系。染料木甙、染料木素、山柰酚和芦丁的检出限分别为0．8，0．6，0．6和1．4mg·L^-1。方法用于分析生槐角和炙槐角样品并做加标回收试验，回收率结果在93．8％～105．2％。Through a thorough study of the working conditions, including the concentration and pH of buffer solution, concentration of β-CD and SDS in the solution and the temperature of capillary, etc., a rapid method of β-CD modified capillary zone electrophoretie determination of genistin, genistein, koenpferol and rutin was proposed. Under the optimum condition, 4 compounds mentioned above were separated nicely within 6 min. Linear relationship between the peak area and concentration of these 4 compounds were obtained in the ranges of 2-400, 2-300, 2-200 and 5-500mg·L^-1 for genistin, genistein, kaempferol and rutin with detection limits of 0. 8, 0. 6, 0. 6 and 1. 4 mg·L^-1 respectively. The proposed method has been applied to the analysis of fruetus sophorae samples, giving recoveries in the range of 93. 8%-105.2%.

关 键 词：区带毛细管电泳 Β-环糊精 染料木甙 染料木素 山柰酚 芦丁 

分 类 号：O657.31[理学—分析化学]