苦瓜子蛋白分离纯化及其理化性质鉴定  

Separation and purification of proteins in seeds of Momordica charantia and identification of their physicochemical characters

作　　者：李璟[1] 温博贵[1] 陈爱云[1] 陈玮莹[1] 吴健宜[1] 李冠武[1] 

机构地区：[1]汕头大学医学院病理系肿瘤分子生物学研究室,广东汕头515041

出　　处：《中草药》2006年第10期1450-1454,共5页Chinese Traditional and Herbal Drugs

基　　金：广东省教育厅自然科学研究项目(Z02041)

摘　　要：目的 建立苦瓜子蛋白分离纯化的方法.方法 应用阳离子交换色谱和凝胶过滤色谱Hiprep 16/10SOURCE 30S和HiLoad 26/60 Superdex 75预装柱从苦瓜种仁中分离纯化苦瓜子蛋白.结果 分离纯化出14个组分的苦瓜子蛋白,它们均为碱性蛋白质,相对分子质量在（1.3～2.9）×104,等电点在9.3～9.6.结论 应用本法能获得供生物活性研究用苦瓜子蛋白组分.Objective A method was developed for the isolation and purification of proteins in the seeds of Momordica charantia. Methods The proteins were isolated and purified from the seeds of M. charantia by a procedure involving extracting with acetones and a series of chromatographies on Hiprep 16/ 10 SOURCE 30S and HiLoad 26/60 Superdex 75. Results Fourteen protein fractions were purified from the seeds of M. charantia. They are all basic proteins with the molecule weight range （1.3—2.9）×10^4 and isoelectric point range 9.3—9.6. Conclusion The bioactive proteins can be purified from the seeds of M. charantia by a procedure involving extracting with acetone and a series of chromatographies on Hiprep 16/ 10 SOURCE 30S and HiLoad 26/60 Superdex 75.

关 键 词：苦瓜 蛋白组分 纯化 

分 类 号：R284.1[医药卫生—中药学]