青海盐湖嗜盐微生物类群及F16菌株生长特性和抗菌、抗肿瘤活性  

Halophilous microbial groups in saline lake of Qinghai and the growth characteristics and anti-microbial and anti-tumor activities of F16.

作　　者：叶央芳[1] 严小军[1] 黄晓春[1] 陈烨[1] 陈海敏[1] 朱世华[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,宁波315211

出　　处：《应用生态学报》2006年第10期1996-1998,共3页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(20472040);教育部新世纪优秀人才支持计划项目(NECT-04-0555);宁波大学科研基金项目(2004617);宁波大学人才基金资助项目.

摘　　要：从青海盐湖底泥中分离出45株嗜盐微生物.其中,丝状真菌F16的抗菌和抗肿瘤活性最强,其发酵液的乙酸乙酯粗提物能抑制4种细菌的生长,尤其对大肠杆菌的抑制作用最强,具有较强的细胞毒活性,当粗提物浓度为50μg.ml-1时对肝癌细胞BEL7402的抑制率可达76.91%.F16菌株的最适生长温度为15℃;培养基盐度升高,对F16的抑制性增强,当盐度超过15%时,F16不生长;在pH值为5～9范围内,F16生长良好.A total of forty-five halophilous microorganisms were isolated from the sediment of saline lake in Qinghai Province, among which, filamentous fungus F16 showed the highest activity of anti-microorganism and anti-tumor. The ethyl acetate extract of F16 culture filtrate showed a strong cytotoxicity, and could inhibit the growth of four kinds of bacteria, especially Escherichia coli. When the concentration of the crude extract was 50 μg · ml^-1, the inhibition rate to liver cancer cell BEL7402 reached 76.91%. The optimal temperature for F16 growth was 15 ℃ ,and the increase of salt concentration in media would inhibit its growth. When the concentration of salt surpassed 15% , F16 could not survive. F16 grew well when the pH value ranged from 5 to 9.

关 键 词：嗜盐微生物 抗菌 抗肿瘤 盐湖 

分 类 号：Q938.8[生物学—微生物学]