仙人掌多糖的提取、分离纯化及GPC法测定其分子量  

Extraction、Isolation and Purification of Polysaccharides in Opuntia and Determination of its molecular weight by GPC method

作　　者：金鑫[1] 赖凤英[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2006年第2期138-140,149,共4页Modern Food Science and Technology

摘　　要：研究了仙人掌多糖水提法的最佳工艺条件,以及仙人掌多糖的分离纯化。试验结果表明,提取液用Savag法脱蛋白后,采用SephacrylS-400柱层析纯化,得到仙人掌多糖OP,经过高效凝胶渗透色谱和常压凝胶柱色谱分析表明,OP为均一多糖,高效液相凝胶色谱法测定OP分子量(Mw)为172591Da。The extraction technology, separation and purification of the polysaccharide from opuntia was studied in this paper. By using Savag method, almost all protein was removed from the extracted juice. Then the polysaccharide was purified by Sephacryl S-400 gelcolumn chromatography, and OP was gained. Its homogeneity is verified by HPGPC and Sephadex G-200 column chromatography. And the molecular weight of OP is 172591Da by the determination of HPGPC.

关 键 词：仙人掌多糖 提取 分离纯化 分子量测定 

分 类 号：TQ28[化学工程—有机化工]