用于叶绿体基因组转化的马铃薯高效再生体系的建立  

The establishment of high efficiency regeneration system of potato chloroplast genomic transformation

作　　者：丁玉梅[1] 杨正安[2] 吴毅歆[1] 周晓罡[1] 张绍松[1] 孙茂林[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所云南省农业生物技术重点实验室,云南昆明650223 [2]云南农业大学园林园艺学院,云南昆明650201

出　　处：《西南农业学报》2006年第B09期98-102,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金项目（2004C0025Q）资助.

摘　　要：云南马铃薯不同品种、不同外植体类型的植株再生能力差异极大。阿诗洋芋、K-18、287、丽薯3号、丽薯4号和紫依的分化率较低，在0-27．8％。多数品种带叶柄的叶片再生能力大于叶盘，茎段分化率最低。会-2是最易诱导植株再生的品种，不同外植体的分化率在20．8％-92．2％，叶盘分化率最高，达92．9％，且平均分化芽数达8．2个，适合于会-2的壮观霉素浓度为100mg／L。筛选出了用于叶绿体转化的马铃薯会-2的高效再生体系和壮观霉素浓度，为Bt基因导入马铃薯叶绿体基因组奠定了基础。The in vitro culture abilities of plant regeneration were extremely different among cultivars and explant types of potato cultivated in Yunnan. The plant regeneration rates which varied from 0 to 27.8% were significantly low among following cultivars： Ashiyangyu, K-18, 287, Lishu3, IAshu4 and Ziyi. Concerning of explant typos, the regeneration frequency of leaf with petiole was higher than those of leaf disc and stem. The cultivar Hui-2 was the easiest one to induce plant regeneration whose plant induction ratio were from 20.8% to 92.9%, the leaf discs with average 8.2 plants per regenerated disc had the highest plant induction proportion （92.9%） . In addition, the spoctinonmycin concentration of 100 mg/L was also selected on cultivar Hui-2 for chimeric aadA gene. This paper established high efficiency regeneration and selection system of potato Hui-2, thus provides a efficient platform for Bt gene transformed into chloroplast genomic of potato.

关 键 词：马铃薯 植株再生 叶绿体转化 外植体 

分 类 号：S532[农业科学—作物学]