胃癌中骨桥蛋白与OCT2的表达及意义  

Study on the expression and significance of OPN and OCT2 in human gastric carcinoma

作　　者：张巧英[1] 姚根有[2] 傅燕萍[3] 赵仲生[4] 张布衣[2] 付琼[2] 

机构地区：[1]绍兴文理学院医学院病理学教研室,绍兴312000 [2]浙江大学病理学与法医学研究所,杭州310031 [3]浙江省绍兴市人民医院病理科,绍兴312000 [4]浙江省人民医院病理科,杭州310014

出　　处：《临床与实验病理学杂志》2006年第5期563-567,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：浙江省科技厅基金资助(No2006C33060)

摘　　要：目的探讨骨桥蛋白(osteopontin,OPN)及转录因子OCT2在胃癌中的表达及其与临床病理参数及预后的关系。方法应用组织微阵列技术和免疫组化SP法检测99例胃癌组织中OPN蛋白与OCT2蛋白的表达情况。结果99例胃癌中OPN蛋白及OCT2蛋白的阳性表达率分别为60·61%、54·55%;OPN蛋白表达与肿瘤大小、浸润深度、脉管侵犯、淋巴结转移及远处转移呈正相关;OCT2蛋白表达也与肿瘤大小、浸润深度、脉管侵犯、淋巴结转移及远处转移呈正相关;OPN蛋白表达与OCT2蛋白表达呈正相关;OPN及OCT2蛋白阳性表达病例的平均生存时间和5年生存率均明显低于阴性表达的病例。结论OPN及OCT2蛋白表达可预测胃癌浸润和转移,是指导临床治疗及评估预后的有价指标。Purpose To study the expression of osteopontin（OPN） and OCT2 in gastric carcinoma and their correlation with clinicpathologic features and survivial time. Methods Tissue microarray （TMA） technique and immunohistochemistry （IHC） were used to detect the expression of OPN and OCT2 in 99 gastric carcinoma specimens. Results lmmunohistochemistry results revealed that： from the 99 cases, the positive rates of OPN and OCT2 protein expression were 60. 61% , 54. 55 % , respectively; there were significant relationships of OPN protein expression with tumor diameter, invasion depth, vessel invasion, lymph node and distant metastasis; there were significant relationships of OCT2 protein expression with tumor diameter, invasion depth, vessel invasion, lymph node and distant metastasis; There was a positive relationship of OPN protein with OCT2 protein expression; the mean survival time in cases with OPN and OCT2 protein positive expression was significantly shorter than those without OPN and OCT2 protein expression. Conclusion The expression of OPN and OCT2 protein can predict the invasion and metastasis of gastric carcinoma; they can be used as markers of prognosis of gastric carcinoma in clinical practice.

关 键 词：胃肿瘤 组织阵列分析 骨桥蛋白 OCT2 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]