大鼠骨髓基质细胞体外分化为内皮细胞静脉移植治疗大鼠局灶脑缺血动物行为学研究  

Assessment of Therapeutic Benefits Treated with Intravenous Administration of Endothelia Cells Derived from Rat Bone Marrow in Rats after Stroke

作　　者：王春雪[1] 姜华[2] 毛淑静[3] 万虹[4] 历俊华[4] 刘丽萍[1] 梁德君[1] 梁宪红[1] 翟晶[4] 王拥军[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经内科 [2]首都医科大学附属北京天坛医院检验科 [3]北京护士学校药理学教研室 [4]北京市神经外科研究所

出　　处：《首都医科大学学报》2006年第5期652-656,共5页Journal of Capital Medical University

摘　　要：目的观察骨髓基质细胞体外诱导分化为内皮细胞后静脉移植治疗大鼠永久大脑中动脉闭塞(MCAO)模型动物行为学改善情况。方法利用高浓度(400μg/L)rhGM-CSF(重组人粒细胞巨噬细胞集落刺激因子)在体外诱导骨髓基质细胞分化为内皮细胞,在细胞移植前进行BrdU(10μmol/L)标记16 h备用。线栓法制作永久SD大鼠MCAO模型(n=45),模型动物随机分为3组:MCAO+移植细胞组(n=15):每只大鼠舌静脉移植3×106的诱导分化后的内皮细胞(相当于0.2 mL细胞悬液);MCAO+移植PBS对照组1(n=15):每只大鼠舌静脉移植0.2 mL的PBS;MCAO对照组2(n=10):血管闭塞后不给予任何干预治疗。在细胞移植前及移植后1、3、5、7、14 d分别采用下列动物行为学评价指标:姿势反射实验,肢体不对称应用试验,角落实验。结果细胞移植组动物行为学评分优于2个对照组,特别是在移植后第7天和第14天较2个对照组差异有统计学意义(P<0.05);细胞移植组在移植后动物行为学评分较移植前有改善,特别是在移植后第7天和第14天较移植后1、3、5 d有显著改善(P<0.05)。结论骨髓基质细胞体外诱导分化后的内皮细胞静脉移植可以改善大鼠局灶脑缺血的神经功能,在移植后第7天开始表现出神经功能的改善,第14天功能改善更明显,其机制尚需进一步研究。Objective To investigate therapeutic benefits with intravenous administration of rat bone marrow derived endothelial cells after stroke and assess the improvement of neurologic function. Methods The endothelial cells derived from bone marrow stromal cells （BMSC） of rats, which were multiplied and differentiated in the medium with high concentration of rhgm- CSF （400μg/L） in vitra, Rats were subjected to permanent cerebral middle artery occlusion （MCAO models, n= 45）, Intravenous injection via tongue vein with 3×10^6 endothelial cells 24 h after stroke for test group（n= 15）; same amount PBS injection for control group 1（n=15）; for control group 2 no intervention after stroke（n= 15）, Neurologic functional behaviour tests including postural reflex test, limb use asymmetrical test and corner test were performed before transplantation and 1, 3, 5, 7, 14 d after stroke, Results Significant recovery of neurological function was found in rats treated with ivadministration of endothelial cells at 7 d and 14 d after stroke, compared with control group 1 and group 2, P〈0.05, Conclusion Endothelial cells derived from rats bone marrow could improve neurological function in rats by intravenous iniection, the effect starts to be significant on the 7 th day after transplantation, and it is even more significant on the 14 th day, P〈0.05, the relevant mechanism should be investigated further in other study,

关 键 词：骨髓基质细胞 内皮细胞 移植 局灶脑缺血 动物行为学 

分 类 号：R743.3[医药卫生—神经病学与精神病学]