C57BL/6小鼠实验性自身免疫性脑脊髓炎模型的建立  

Establishment of experimental autoimmune encephalomyelitis model in C57BL/6 mice

作　　者：王菊蓉[1] 张冉[1] 王栋[1] 邱磊[1] 郭满盈[1] 弓雪莲[1] 郭葆玉[1] 

机构地区：[1]第二军医大学药学院生化药学教研室,上海200433

出　　处：《第二军医大学学报》2006年第10期1089-1091,共3页Academic Journal of Second Military Medical University

摘　　要：目的:建立实验性自身免疫性脑脊髓炎(EAE)的小鼠模型,为探讨多发性硬化的免疫病理机制和实验性治疗提供依据。方法:C57BL/6小鼠40只,分为3组。EAE组25只,采用皮下注射完全弗氏佐剂与300μg MOG35-55制备的乳化抗原,辅以腹腔注射109个/ml百日咳疫苗的方法诱导发病;佐剂组8只,用生理盐水代替抗原肽制备免疫乳剂,余同EAE组;正常对照组7只,仅注射生理盐水。采用H-E和LFB染色的方法观察小鼠EAE模型的组织学改变。结果:从免疫后11 d开始,EAE组小鼠有22只陆续出现EAE临床症状,发病率为88%。佐剂组与正常对照组小鼠均未出现临床神经系统症状。光镜下可见EAE组小鼠的大脑和脊髓组织中有大量的炎性细胞浸润,白质脱髓鞘改变明显。结论:此种造模方法简单可行,模型稳定,可在国内广泛推广。Objective：To establish an experimental autoimmune encephalomyelitis （EAE） model in C57BL/6 mice, providing a basis for studying the immunopathological mechanism and experimental therapy of multiple sclerosis. Methods： Twentyfive C57BL/6 mice were immunized with both 300μg MOG35-55 in complete Freund＇s adjuvant （CFA） and 10^9/ml Bordetella pertussis vaccine （BPV） to establish EAE model. Mice in adjuvant group （n=8） were treated with CFA, BPV and normal saline; mice in control group （n= 7） were treated with normal saline. The pathologic changes of the central nervous system were studied by H-E staining and Luxol Fast Blue （LFB） staining. Results： The clinic symptoms of EAE were present in 22 mice in model group since the 11^th day post-immunization, with the incidence being 88%. There was no evident EAE symptom in CFA group and control group. Light microscopy showed there were abundant inflammatory cells infiltrated in the cerebral and spinal cord tissues in EAE mice, with evident demyelination in white matter. Conclusion： This method is easy to be carried out; the model established by it is stable and worth popularizing.

关 键 词：脑脊髓炎 自身免疫性 实验性 模型 动物 

分 类 号：R593.2[医药卫生—内科学]