IV型胶原酶在不同转移潜能的前列腺癌细胞系中的表达及活性分析  

Type IV Collagenase Expression in Human Prostate Carcinoma Cell Lines with Different Metastasis Potentials

作　　者：张林琳[1] 贺大林[1] 李翔[1] 李磊[1] 朱国栋[1] 王新阳[1] 

机构地区：[1]西安交通大学医学院第一附属医院泌尿外科,陕西西安710061

出　　处：《中华男科学杂志》2006年第10期913-916,共4页National Journal of Andrology

摘　　要：目的:探讨前列腺癌细胞转移潜能与IV型胶原酶基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)的关系。方法:应用免疫组织化学SABC法,研究IV型胶原酶在不同转移潜能的前列腺癌细胞株LNCaP、C4-2B中表达变化。同时采用明胶酶谱法对这2种前列腺癌细胞系分泌的IV型胶原酶活性进行分析。结果:①免疫组织化学染色显示,LNCaP、C4-2B细胞中均有MMP-2、MMP-9表达,主要表达于细胞质中,C4-2B细胞表达明显强于LNCaP(P<0.01)。②人前列腺癌细胞LNCaP的条件培养液中仅能检测到极低的MMP-2和MMP-9活性,酶谱条带的积分A值分别为0.89±0.02和0.86±0.01,主要以酶原形式存在,而C4-2B细胞则表现较强的MMP-2、MMP-9活性,积分A值分别为96.32±4.36和33.89±1.84,其分泌的MMP-2则以活性形式为主,其分泌量显著高于LNCaP细胞(P<0.01)。结论:前列腺癌细胞中IV型胶原酶的表达和分泌,有助于癌细胞的侵袭和转移;前列腺癌细胞的侵袭转移潜能与其产生IV型胶原酶的能力密切相关。Objective ： To investigate the correlation between type Ⅳ collagenase expression and metastatic potential of human prostate carcinoma cells. Methods： Human prostate carcinoma cell lines （LNCaP and C4-2B） with different metastatic potentials were selected. Using SABC immunocytochemical staining and zymographic analysis, we detected the difference of type Ⅳ collagenase expression and activity between the two cell lines. Results ： The expression of type IV coHagenase （ MMP-2, MMP-9） could be detected in both LNCaP and C4-2B, mainly in cytoplasm, much higher in C4-2B than in LNCaP （P 〈0.01 ）. The activities of type Ⅳ collagenase in the conditioned medium of prostatic carcinoma cell LNCaP was the lowest, and the quantitative estimation values of MMP-2 and MMP- 9 were 0.89 ±0.02 and 0.86 ±0.01, respectively. However, the quantitative estimation values of MMP-2 and MMP-9 in the condi- tioned medium of C4-2B were 96.32 ±4.36 and 33.89 ± 1.84, respectively. Compared with LNCaP, the activity of type Ⅳ collagenase in C4-2B was much higher （ P 〈 0.01 ）. Conclusion ： Compared with LNCaP, the higher production capability of type IV colla-genase in CA-2B may possibly contribute to its invasive and metastatic potentials. The expression of type Ⅳ collagenase of human prostate carcinoma cells is closely correlated to the metastatic and invasive potential.

关 键 词：前列腺癌 肿瘤转移 肿瘤侵袭 Ⅳ型胶原酶 

分 类 号：R737.25[医药卫生—肿瘤]