尾叶香茶菜二萜类化合物F对肝癌细胞株SMMC-7721的影响  

Apoptosis in SMMC-7721 cells induced by diterpenoid F derived from rabdosia excise.

作　　者：孙明莉[1] 孙晓娟[2] 张宏宇[3] 孔晓霞[3] 袁兆新[3] 穆桂芳[3] 李扬[3] 王冠军[1] 李洪岩[3] 

机构地区：[1]吉林大学第一医院急救医学科,吉林长春130021 [2]吉林大学中日联谊医院妇产科,吉林长春130000 [3]吉林大学基础医学院病理生理学教研室,吉林长春130021

出　　处：《临床肝胆病杂志》2006年第5期344-346,共3页Journal of Clinical Hepatology

基　　金：吉林省科技厅医学专项基金(2006-0271)

摘　　要：观察尾叶香茶菜中提取物二萜类化合物F对人肝癌细胞株SMMC-7721的作用并探讨其机制。分别应用MTT法、AO／EB荧光染色法分析细胞存活率和凋亡情况,利用DCFH-DA荧光分光光度法检测细胞内过氧化物(ROS)的水平。二萜类化合物F抑制SMMC-7721细胞增殖,且呈时间剂量依赖性,二萜类化合物F作用的SMMC-7721细胞内ROS水平明显升高。二萜类化合物F可以启动细胞内氧化应激诱导SMMC-7721细胞坏死或凋亡而抑制肝癌细胞株增殖。To determine the apoptosis of human hepatoma carcinoma cell line SMMC -7721 induced by diterpenoid F derived from radbosia excise, and to study its mechanism. The survival and apoptotic rate of SMMC -7721 cells were detected by MTT and AO/EB fluorescent staining respectively. And the intracellular ROS level was analyzed by fluorospectrophotometry. Diterpenoid F could inhibit the proliferation of SMMC -7721 in a dose -and time -dependent manner. The intracellular ROS increases in SMMC -7721 through oxidative stress pathway.

关 键 词：二萜类化合物F 尾叶香茶菜 肝癌细胞株 氧化应激 

分 类 号：R735.7[医药卫生—肿瘤]