p33^(INGI)、p53在结直肠癌中的表达及其相互关系  

Expression of p33^(ING1) and p53 in colorectal carcinoma and their relationship between each other

作　　者：梁君林[1] 周永淳[1] 陈利生[1] 

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,南宁530021

出　　处：《肿瘤》2006年第10期924-926,共3页Tumor

基　　金：广西科学基金项目(编号:桂科回0342018);教育部留学回国启动基金(编号:教外司留2004-176)

摘　　要：目的:探讨p33ING1、p53在结直肠癌中的表达及其相互关系。方法:应用免疫组化SP法检测60例结直肠癌组织及相应正常黏膜组织中p33ING1、p53的表达。结果:结直肠癌组织、相应正常黏膜组织中p33ING1蛋白的阳性表达率分别为43．3％(26／60)、100％(60／60)(P<0．01),p53蛋白分别为51．6％(31／60)、0％(0／60)。p33ING1在无淋巴结转移组及淋巴结转移组癌组织中的阳性表达率分别为57．6％(19／33)、25．9％(7／27)(P<0．05);在Dukes A、B期、Dukes C、D期病例中分别为56．7％(17／30)、30．0％(9／30)(P<0．05)。在p53表达阴性的29例中有12例(41．4％,12／29)p33ING1表达缺失,而p53阳性的31例病例中有22例(71．0％,22／31)p33ING1表达阴性(P<0．05),在p53蛋白表达阳性的病例中p33ING1蛋白表达明显缺失,两者表达呈负相关。结论:p33ING1在结直肠癌组织中低表达,与p53互相协同,在结直肠癌的发生、发展中可能起重要作用。Objective： To investigate the expressions of p33^ING1 and p53 and their relationship in colorectal carcinoma （CRC）. Methods： The expressions of p33^ING1 and p53 protein were examined in 60 cases of colorectal cancerous tissue and their corresponding normal mucous tissues by immunohistochemical SP method. Results： The positive expression rates of p33^ING1 were 43.3%（26/60） and 100% （60/60） in CRC and normal mucous tissues, respectively. The positive expression rates of p53 protein were 51.6% （31/60） and 0% （0/60） in CRC and normal mucous tissues, respectively. The positive rates of p33^ING1 expression were 57.6% （19/33） and 25.9% （7/27） in cancerous tissues with or without lymph node metastasis （P〈0.05）. The positive rates of p33^ING1 expression in cancerous tissues at Dukes＇ A and B stages were significantly higher than that at Dukes＇ C and D stages [56.7% （17/30）vs 30.0% （9/30）]. The expression of p33^ING1 expression was negative in 12 out of 29 p53-negative patients （41.4%）. The negative rate was increased to 71.0% （22/31） in p53-positive patients. Conclusion： Low expression of p33^ING1 synergied with p53 may play an important role in the carcinogenesis and development of CRC.

关 键 词：结肠直肠肿瘤 基因 ING1 P53蛋白 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]