慢性淋巴细胞白血病细胞化学染色的特点分析  

Analysis of characteristics with regard to cytochemical staining patients with chronic lymphocyte leukemia

作　　者：崔雯[1] 许议丹 孙万臣[1] 王慧君[1] 陈辉树[1] 薛艳萍[2] 肖志坚[2] 邱录贵[2] 王建祥[2] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所,血液病医院检验科,天津300020 [2]中国医学科学院中国协和医科大学血液学研究所,血液病医院血液内科,天津300020

出　　处：《白血病．淋巴瘤》2006年第5期341-343,共3页Journal of Leukemia & Lymphoma

摘　　要：目的应用细胞化学染色方法诊断慢性淋巴细胞白血病(CLL)。方法用9种细胞化学染色方法对75例CLL患者骨髓片进行染色分析。结果CLL的骨髓过氧化物酶(MPO)和苏丹黑染色(SBB)为阴性;中性非特异性酯酶(NSE)和α-丁酸酯酶(NBE)染色为弱阳性,糖原(PAS)染色均为阳性,阳性物呈细颗粒、中粗颗粒、粗颗粒散在分布;特异性酯酶(CE)染色4%可见阳性,阳性率均低于6%;酸性磷酸酶(AcP)染色呈弱阳性,13.3%抗酒石酸酸性磷酸酶(TRAP)染色可见阳性,以(+)为主。结论CLLCE染色大部分阴性,个别病例可见阳性(6%),但阳性率明显低于大颗粒淋巴细胞白血病(LGLL)(46.9%);AcP染色呈弱阳性,低于LGLL,非霍奇金淋巴瘤(NHL)和HCL;TRAP染色少部分病例虽可见阳性(13.3%),但阳性率及强度明显低于毛细胞白血病(HCL),NHL和幼淋细胞白血病(PLL)。Objective To investigate the utilities of cytochemical staining technique in diagnosis of Chronic lymphocytic leukemia（CLL）. Methods Nine kinds of cytochemical stains were performed. Results MPO and SBB staining are negative in patients with CLL. NSE and NBE staining are weak positive. PAS staining is positive and the positive contents are appeared by fine and moderate thick and thick particles distributing diffusely. 4 % of CE staining may be positive and positive rate is less than 6 %. ACP staining is weak positive. 13.3 % of TRAP staining may be positive and most of the results are ＂＋＂. Conclusion CE staining is almost negative in patients with CLL. Few of CE may be positive （6 %）. But positive rate is less than the rate of patients with large granular lymphocytes leukemia（LGLL）（46.9 %）. ACP staining is weak positive and the rate is obviously less than the rate of LGLL, non-Hodgkin lymphoma （NHL） and hairy cell leukemia （HCL）. Few of TRAP staining may be positive（13.3 %） in cell. Neither the positive rate nor intensity is obviously less than that in the patients with HCL, NHL and prolymphocytic leukemia（PLL）.

关 键 词：慢性淋巴细胞白血病 细胞化学染色 

分 类 号：R733.72[医药卫生—肿瘤]