川陈皮素诱导非小细胞肺癌A549细胞凋亡的研究  

Inhibition of nobiletin on human non-small cell lung cancer cell line A549

作　　者：管晓琳[1] 罗刚[1] 朱玲[1] 周黎明[1] 

机构地区：[1]四川大学华西医学中心药理学教研室,成都610041

出　　处：《中国药科大学学报》2006年第5期443-446,共4页Journal of China Pharmaceutical University

基　　金：四川省科技厅应用基础研究计划资助项目(No.02SY029-137)~~

摘　　要：目的:探讨川陈皮素(5,6,7,8,3′4-′hexamethoxyflavone)促非小细胞肺癌A549细胞凋亡的作用。方法:以不同浓度的川陈皮素作用于A549细胞,分别用细胞生长曲线、集落形成抑制实验研究川陈皮素对A549的增殖抑制作用,Hoechst 33258荧光染色观察细胞核形态变化,DNA琼脂糖凝胶电泳检测细胞凋亡,流式细胞术观察细胞周期改变。结果:生长曲线提示川陈皮素对A549细胞的抑制作用呈明显的时效和量效关系;集落形成抑制实验表明:药物浓度10μg/mL^40μg/mL对A549集落形成抑制率为10%~50%。Hoechst 33258染色后细胞、细胞核出现明显的凋亡形态学变化;DNA琼脂糖凝胶电泳出现典型DNA梯状条带。流式细胞仪检测到细胞周期阻滞于G2/M期,G0/G1期细胞明显减少;随着剂量的增加凋亡率明显增高。结论:川陈皮素可以诱导非小细胞肺癌A549细胞凋亡。Aim：To investigate the inhibitory effect of nobiletin （5, 6, 7, 8, 3＇4＇-hexamethoxyflavone） on human non-small cell lung cancer cell line A549 and its mechanism of action. Methods： The inhibitory effect of nobiletin on the proliferation of A549 ceils was evaluated by the growth curve and the clone-forming assay following application of nobiletin to the ceil line. The possible apoptosis-inducing effect was assessed by the morphological observation under microscope （Hoechst 33258 fluorescent staining）, flow cytometric analysis and agarose gel electrophoresis. Results： It was found that the growth curve of the cell line indicated the dose-effect and time-effect relationships. Estimation of the inhibiting rate of nobiletin to A549 in clone-forming assay ranged from 10% to 50% when nobiletin solution of ranging from 10μg/mL to 40μg/mL was applied. The characteristic morphology typical for apoptosis was indicated under the microscopic observation. DNA zone indicative of the apoptosis was shown on agarose gel electrophoresis. The ceil cycle was arrested in G2/M phase meanwhile it was found that cells in Go/G1 phase decreased and the percentage of apoptosis increased as dose size increased. Conclusion： Nobiletin could inhibit the growth of A549 cellsin vitro, and its mechanism might probably be associated with the apoptosis.

关 键 词：川陈皮素 A549细胞 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]