成都地区汉族群体ApoB3’位点扩增片段长度多态性研究  

ENGLISH OF ORIGINAL ARTICLES ( Study on Length Polymorphology of Enlarging Fragment at Apo B 3 Locus in Population Cohort of Han Nationality in Chengdu Region )

作　　者：景强[1] 孙光云[1] 吴梅筠[1] 

机构地区：[1]华西医科大学法医学系

出　　处：《中国输血杂志》1996年第4期174-177,共4页Chinese Journal of Blood Transfusion

基　　金：卫生部科研基金

摘　　要：用扩增片段长度多态性分析技术对成都地区汉族群体２００名无关个体ＡｐｏＢ３’位点扩增片段长度多态性进行了分析，查出１６个等位基因，片段大小在５４２～９９２ｂｐ之间，基因频率０００２５～０３７５０，杂合性为０７９，ＤＰ值０９５。在１３８种可能的基因型中，发现了４３种基因型，基因型的观察值与期望值符合ＨａｒｔｄＷｅｉｎｂｅｒｇ平衡定律（χ２＝２１３，ｄｆ＝１０，Ｐ＞００１），家系调查证明ＡｐｏＢ３’位点是按孟德尔定律遗传的。采用ＨＳＰＣＲ法提高了方法的灵敏度和特异性。The length polymorphology analysis of enlarging fragment at Apo 3 locus in 200 unrelated patients out of population cohort of Han nationality in Chengdu region has been performed,using the technique of analysing the length polymorphology of enlarging fragment.As a result,sixteen alleles have been found,with their fragment sizes ranging between 542 and 992 bp,gene frequencies of 0.0025～0.3750,heterozygosity of 0.79 and DP value of 0.95.Of 138 possible genotypes,43 genotypes have been found.The observed and expected values of those genotypes coincide with the Hardy Weinberg equilibrium( χ 2=2,13,df=1.0,P >0.01).The family line investigation showed that the inheritance of Apo B 3 locus follows the Mendelian Law.The sensetivity and specificity of the technique can be improved with HS PCR method.

关 键 词：成都 汉族 ApoB3'位点 扩增片段 长度 多态性 

分 类 号：R89[医药卫生—法医学]