防龋DNA疫苗pGJA-P/VAX免疫小鼠后的抗DNA抗体检测  

Detection of Anti-dsDNA Antibodies and Anti-ssDNA Antibodies in Mice after Immunized with pGJA-P/VAX

作　　者：杜宇[1] 樊明文[1] 李宇红[1] 郭继华[1] 边专[1] 陈智[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,湖北武汉430079

出　　处：《口腔医学研究》2006年第5期465-467,共3页Journal of Oral Science Research

基　　金：国家自然科学基金资助重点项目(编号:30330660)

摘　　要：目的:检测防龋DNA疫苗pGJA-P/VAX经鼻腔免疫和肌肉免疫后是否会产生抗DNA抗体。研究该疫苗是否会导致自身免疫性疾病。方法:提取纯化的质粒pGJA-P/VAX分别经鼻腔和肌肉免疫小鼠,于第0、2、4、8周取血清做抗DNA抗体检测实验(ELISA法)。结果:各实验组之间均无显著性差异,各组免疫前后及各组间比较(P>0.01)。结论:说明DNA疫苗pGJA-P/VAX及其载体pVAX1对小鼠均不会引起自身免疫反应,导致自身免疫病。Objective： To compare the levels of anti -ds -DNA antibodies and anti -ss -DNA antibodies in mice immunized with pGJA - P/VAX and mice without such treatment. Methods ： pGJA - P/VAX was administrated imtramuscularly or intranasally to mice, and all mice received a booster immunization 2 weeks later after the initial immunization. The concentrations of anti - ssDNA antibodies and anti - dsDNA antibodies of unimmunized mice were measured by the method of ELISA, which were used as baseline for the comparison study. 2, 4 and 8 weeks after the initial immunization, blood samples of these immunized mice were collected to check the levels of anti - ssDNA antibodies and anti - dsDNA antibodies by using the same method. Results ： No statistically significant changes of anti - ssDNA antibodies and anti - ds - DNA antibodies were found between unimmunized and immunized mice. Conclusion ： The blood levels of anti - ss - DNA antibodies and anti - ds - DNA antibodies did not statistically significantly increase by the repeated and high dose adminstration of anticaries DNA vaccine pGJA - P/VAX. Additionally, autoimmune diseases were found among the immunized mice at the specific experiment condition.

关 键 词：龋齿 疫苗 DNA ds—DNA ss—DNA 

分 类 号：R781.1[医药卫生—口腔医学]