口腔鳞癌引流区淋巴结中树突状细胞特征性表型的免疫组化分析  

Immunohistochemical Analysis of Characteristic Phenotype of Dendritic Cell in Draining Lymph Node of Patients with Oral Squamous Cell Carcinoma

作　　者：王志勇[1] 胡勤刚[1] 黄晓峰[1] 韩伟[1] 杨旭东[1] 

机构地区：[1]南京大学医学院附属口腔医院口腔颌面外科,江苏南京210008

出　　处：《口腔医学研究》2006年第5期482-485,共4页Journal of Oral Science Research

摘　　要：目的:探讨OSCC的DLN中DC的功能状态。方法:免疫组化标记OSCC的DLN和扁桃体组织中DC的CD1 a,CD83分子。统计CD1 a+DC和CD83+DC的浸润程度,以及CD83+DC/CD1 a+DC的比值。结果:CD1 a+DC在OSCC的DLN中的浸润程度显著高于扁桃体组织(P<0.01),在鳞癌转移的淋巴结中显著低于未转移的淋巴结(P<0.01)。CD83+DC浸润程度在OSCC的DLN中明显低于扁桃体(P<0.01),但鳞癌转移的淋巴结与未转移者无显著差异(P>0.05)。OSCC的DLN中CD83+DC/CD1 a+DC的比值显著低于扁桃体组织(P<0.05)。结论:OS-CC的DLN中,DC以不成熟DC为主,难以实现其激活T淋巴细胞反应的能力。Objective： To evaluate the functional state of dendritic cell （DC） in the draining lymph nodes of patients with oral squamous cell carcinoma. Methods： Immunohistochemistry was used to detect CD1 a and CD83 molecules expressed by DC in paraffin embedded tissues from 34 - case draining lymph nodes of OSCC and 10 - case tonsils. DC counts, representing the density of DC infiltrating in the tissues, was performed under high - power field. The densities of DC expressing CDla and CD83 molecules, and the ratio of CD83^＋ DC/CD1a^＋ DC were compared between two groups. Results： There were CD1a^＋ DCs in all cases. The density of CD1a^＋ DCs in the group of draining lymph node was significantly higher than the one of tonsil （ P 〈0.01 ）, but it was lower in draining lymph nodes with metastasis than without metastasis. The density of CD83^＋ DC was significantly lower in draining nodes than tonsils （ P 〈0.01 ）, but there was no significant difference between the nodes with metastasis and without metastasis（P 〉0.05）. The ratio of CD83^＋ DC/CD1a^＋ DC was significantly higher in draining lymph nodes （P 〈 0.05 ） than tonsils. Conclusion： Immature DC was predominant in the draining lymph nodes of OSCC, and the functional mature of DC was blocked so that DC could have difficulty in activating T lymph cells.

关 键 词：树突状细胞 口腔鳞癌 免疫组化 淋巴结 

分 类 号：R739.8[医药卫生—肿瘤]