新生大鼠缺氧缺血时脑皮质Fas-L的变化  

Changes of Fas-L Protein Expression in the Cortex of Neonatal Rats with Hypoxic-ischemic Brain Damage

作　　者：杨秀芳[1] 陈敬国[1] 蒋犁[2] 

机构地区：[1]中山市人民医院儿科重症监护室,广东中山528403 [2]东南大学医学院附属中大医院,江苏南京210009

出　　处：《贵阳医学院学报》2006年第5期416-417,共2页Journal of Guiyang Medical College

基　　金：中山市科技计划资助项目(2005A106)

摘　　要：目的:探讨缺氧缺血脑损伤时脑皮质Fas-L蛋白表达的变化。方法:建立新生儿缺氧缺血性脑损伤(HIBD)动物模型并设假手术组作为对照,根据检测需要,在缺氧、缺血后24h,3d,7d应用免疫组织化学SP法测定脑皮质Fas-L蛋白表达情况。结果:左脑皮质Fas-L蛋白表达阳性率假手术组、实验组24h依次为8.04±3.50,120.20±6.10;3d依次为8.20±3.10,87.6±5.90;7d依次为8.01±2.80,8.32±2.90。实验组与假手术组比较,在第7天时,差异无显著性(P>0.05);24h,3d时明显增多,差异显著(P<0.05)。结论:新生大鼠HIBD后24h,3d,7d结扎侧脑皮质,Fas-L蛋白开始表达增高,后降低。Objective： To explore the changes of the expression of Fas-L protein in cortex of newborn rats with hypoxic-ischemic brain damage （HIBD）. Methods： HIBD animal model was established with rats as experiment group, and sham operation group was set up as control. The contents of Fas-L protein in cortex were detected in 24 hours, 3 days and 7 days after the episode of hypoxia and ischemia by using immunohistochemistry method. Results： Comparing with the sham operation group, the expression of Fas-L protein （ 24 h and 3 d） was obviously stronger in the brain cortex of the ligated side in experiment group （ P 〈 0. 05 ） , but there was no significant difference between the expression of the two groups on the seventh day. Conclusion： The expression of Fas-L protein in cortex of newborn rats increases rapidly in the beginning of HIBD and decreases latter.

关 键 词：缺氧缺血 脑 免疫组织化学 大鼠 SPRAGUE-DAWLEY FAS-L蛋白 

分 类 号：R748.02[医药卫生—神经病学与精神病学] R722.120.2[医药卫生—临床医学]