反义抑制PTTG对胆管癌细胞株QBC939中VEGF表达的影响  

The inhibitory expression of VEGF in antisense blocking of PTTG gene on human cholangiocarcinoma cell line QBC939

作　　者：陈鸰[1] 汤恢焕[1] 冯超[1] 文言广[1] 菅志远[1] 

机构地区：[1]中南大学湘雅医院普通外科,湖南长沙410078

出　　处：《中国普通外科杂志》2006年第10期752-756,共5页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(No.20335020);湖南省卫生厅科研基金资助项目(No.B2005011)

摘　　要：目的探讨抑制垂体瘤转化基因(PTTG)表达后对血管内皮生长因子(VEGF)表达的影响。方法将已构建好的反义PTTG真核表达载体pcDNA 3.1-PTTGas按浓度梯度转染至胆管癌细胞株QBC 9 3 9中,培养4 8 h后利用RT-PCR和W estern B lot方法检测PTTG和VEGF表达量的变化,并将两者的变化行直线相关分析。结果成功转染反义PTTG至QBC 9 3 9中;转染后PTTG和VEGF在mRNA水平最大抑制率分别为4 0.6 2%和3 4.1 4%;在蛋白质水平分别为5 5.5 5%和3 8.4 6%。在mRNA和蛋白质两个层面上均下降,呈线性正关系(r=0.9 7 0,0.9 8 5,P<0.0 5)。结论反义阻断胆管癌细胞株中的PTTG表达能使VEGF的表达下降,下降程度呈正相关。Objective To investigate the effect of expression of pituitary tumor transforming gene （ PTTG ） on the expression of inhibitory of vascular endothelial growth factor （VEGF）. Methods The constructed recomibinant vector, pcDNA3. 1-PTTGas that contained full-length antisense PTTG, was transfected into the cholangiocarcinoma callline QBC939 in different quantity. After 48h incubation, the variation of expressions of PTTG and VEGF mRNAs and proteins were observed by RT-PCP and Western-blot method. Results After successful transfecting the recomibinant vector into QBC939, the mRNAs and proteins of PTTG and VEGF were all inhibited. Linear correlation analysis showed positive correlation between descendent extents of PTTG and VEGF （ P 〈 0. 05 ）. Conclusions Antisense blocking PTTG gene can inhibit the expression of VEGF in both mRNA and protein levels, and a positive correlation between descendent extents of PTTG and VEGF was observed.

关 键 词：血管内皮生长因子 垂体瘤转化基因 肿瘤细胞 培养的 胆管肿瘤/遗传学 

分 类 号：R735.8[医药卫生—肿瘤] Q58[医药卫生—临床医学]