细胞因子诱导的杀伤细胞对胆囊癌细胞株GBC-SD杀伤活性的影响  

Effect of killer cell induced by cytokines on the killing activity to GBC-SD gallbladder cancer cell strain

作　　者：任宏[1] 胡恒通[1] 刘刚[2] 杜宁[1] 田伟[1] 邓智平[1] 石景森[3] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤外科,陕西西安710061 [2]西安交通大学医学院第一附属医院普通外科,陕西西安710061 [3]西安交通大学医学院第一附属医院肝胆外科研究室,陕西西安710061

出　　处：《肝胆胰外科杂志》2006年第5期282-285,共4页Journal of Hepatopancreatobiliary Surgery

摘　　要：目的研究脐血单个核细胞体外多向诱导分化为LAK,CD3AK,CIK细胞及体外对胆囊癌细胞株GBC-SD杀瘤活性的变化。方法采用IL-2刺激诱生LAK细胞;用CD3单抗和IL-2刺激诱生扩增CD3AK细胞;加入IFN-γ,24h后加入IL-1,CD3单抗,IL-2刺激诱生CIK细胞,观察它们的扩增情况,并分析其相互关系;取培养的CIK细胞用流式细胞仪分析表型;用MTT法以胆囊癌细胞株GBC-SD为靶细胞,分组测定LAK,CD3AK,CIK细胞的杀伤活性。结果CD3AK和CIK细胞的扩增能力远大于LAK细胞(P<0.05)。CIK细胞和CD3AK相比,在前期无明显差异,至第20天左右时CIK细胞的扩增倍数显著高于CD3AK(P<0.05)。流式细胞仪分析结果显示CIK细胞培养后,其主要效应细胞CD3+CD56+细胞较培养前显著增加(P<0.05),CD8+细胞也较培养前显著增加(P<0.05)。CD3AK和CIK细胞对胆囊癌细胞株GBC-SD的杀伤活性均显著高于LAK细胞(P<0.05),而CIK细胞杀伤活性又强于CD3AK细胞(P<0.05)。结论CIK细胞具有更高的扩增能力和更强的杀伤活性,是肿瘤过继免疫治疗中更为有效的杀伤效应细胞。Objective To study multi-direction derivation from cord blood mononuclear cells to CD3AK, LAK and CIK ceils as well as variations of killing activity to the GBC-SD gallbladder cancer cell strain in vitro. Methods CD3mAb and IL-2 were used to induce CD3AK cells; IL-2 was used to induce LAK cells; IFN-γ was used in the beginning, then IL-1, CD3mAb and IL-2 were used to induce CIK cells after 24 h for observing amplification status and analysis of the relationship; cultivated CIK ceils were taken to analyze phenotype by flow cytometer, subsequently, the GBC-SD gallbladder cancer cell strain was taken as target cells to evaluating killing activity of CD3AK, LAK and CIK cells, respectively by MTT method. Resulis The amplification activity of CD3AK and CIK cells were all far higher than LAK ceils （ P 〈 0.05）. Compared with CD3AK cells, the amplification activity of CIK cells had no obviously difference at prophase, but was far higber than CD3AK cells at about 20 d （ P 〈 0.05）. The analytic results of flow cytometer disclosed that the amount of CD3^＋ CD56^＋ cells which were major effector cells after CIK cells being cultivated was far higher than before （ P 〈 0.05）, moreover, the amount of CD8^＋ calls was far higher than before as well （P 〈0.05）. The killing activities of CD3AK and CIK cells to the GBC-SD gallbladder cancer cell strain were all far higher than LAK cells, besides, killing activity of CIK cells was far higher than CD3AK cells （ P 〈 0.05）. Conclusion CIK cells have higher amplification activity and higher killing activity,which can be taken as morn effective killing cells applied to the tumor adoptive immunotherapy.

关 键 词：CD3AK细胞 LAK细胞 CIK细胞 增殖能力 杀伤活性 胆囊肿瘤 

分 类 号：R735.8[医药卫生—肿瘤]