苯乙酸对胶质瘤细胞中HoxA10基因mRNA表达的影响  

The Effect of Phenylacetate on mRNA Expression of HoxA10 Gene in Glioma Cell Line C6

作　　者：杜超[1] 潘玉琢[2] 田宇[1] 董震[3] 

机构地区：[1]吉林大学中日联谊医院神经外科,长春市130033 [2]吉林大学白求恩医学院基础医学部病理生理教研室 [3]吉林大学中日联谊医院耳鼻喉科

出　　处：《中国肿瘤临床》2006年第20期1150-1152,1160,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30271334;39900152);中国博士后科学基金(编号:2003034028);吉林省科学技术厅科学基金项目资助(编号:200506161)

摘　　要：目的:观察苯乙酸(PA)诱导分化胶质瘤细胞C6过程中,同源盒基因HoxA3和HoxA10mRNA水平表达的变化。方法:应用逆转录PCR(RT-PCR)及图像分析法,分组检测原代培养的大鼠星形胶质细胞中及应用PA前后胶质瘤细胞C6中HoxA3和HoxA10基因mRNA水平表达。结果:HoxA3基因在正常大鼠脑组织和应用PA前后的胶质瘤C6细胞中,均存在明显表达,图像分析显示各组间差异无显著性。HoxA10基因在正常大鼠脑组织中未见表达;在胶质瘤C6细胞中存在明显表达;应用0.5mmol/LPA后,胶质瘤C6细胞中HoxA10基因弱表达,与未用药的C6细胞比较,图像分析显示差异有显著性(P<0.05);应用1.0mmol/LPA后,HoxA10基因未见表达,与未用药的C6细胞比较,图像分析显示差异有显著性(P<0.01)。结论:苯乙酸抑制胶质瘤细胞增殖的作用机理可能与降低胶质瘤细胞HoxA10基因mRNA水平表达有关。Objective： To observe the change of mRNA expression of HoxA3 and HoxA10 gene treated by phenylacetate （PA） in glioma C6 cells. Methods： Astrocytes of rat brain tissue were primarily cultured and C6 cells were cultured in vitro. The expression of HoxA3 and HoxA10 mRNA were detected by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） in the presence of specific primers. The difference of Hox gene expression level between before and after treated by PA in C6 cells was analyzed by student t-test. Results： HoxA3 gene had significantic expression in rat brain astrocyte cells and glioma C6 cells before and after treated by PA. HoxA10 gene had no expression in rat brain tissue. There is significantic expression of HoxA10 gene in glioma C6 cells. The expression of HoxA10 gene became weaker in glioma C6 cells after treated by 0.5 mM PA （P〈0.05）, HoxAlO gene had no expression after treated by 1.0 mmol/L PA in glioma C6 cells （P〈0.01）. Conclusion： The mechanism of PA action may be correlated with down-regulatated mRNA expression of HoxA10 gene.

关 键 词：苯乙酸 胶质瘤 HOXA10基因 

分 类 号：R739.41[医药卫生—肿瘤]