Caveolin-1对MGC803细胞增殖的影响及机制  

The effect and mechanism of caveolin-1 upregulation on the proliferation of MGC803 cell line

作　　者：罗红梅[1] 唐圣松[2] 谭力铭[3] 廖端芳[2] 严鹏科[2] 刘月顺[1] 龙治峰[1] 

机构地区：[1]南华大学组胚教研室,湖南衡阳421001 [2]南华大学药物药理研究所 [3]湖南省怀化市第一人民医院临床药学室

出　　处：《中国医师杂志》2006年第10期1323-1325,共3页Journal of Chinese Physician

基　　金：国家自然科学基金(30270684);湖南省杰出中青年专家专项基金(02JJYB004)资助项目

摘　　要：目的研究caveolin-1对胃癌细胞生物学行为的影响及作为基因治疗的候选基因可能性。方法用L ipofectin将caveolin-1基因稳定转染胃癌细胞系建立稳定表达caveolin-1的胃癌细胞系,并将空载体转染MGC803作为对照。采用western b lot检测转染及未转染的MGC803细胞中caveolin-1的表达情况。应用细胞计数、流式细胞术等检测caveolin-1对MGC803细胞生长的影响。结果转染后的MGC803细胞的caveolin-1的表达明显高于未转染细胞;细胞的群体倍增时间明显延长,细胞增殖周期明显延长,且阻滞于G0/G1期。结论Caveolin-1能抑制MGC803细胞的增殖,且将其阻滞于G0/G1期。Objective To study the effect of caveolin-1 gene expression on the proliferation of human gastric adenocarcinoma cells, and to explore the possibility for its future usage in gene therapy. Methods The full-length caveolin-1 gene was stably transfeeted into the MGC803 cell line by lipofectin. The Pcl neo vector was transfected at the same time as mock control, The expression of caveolin-1 was detected by Western blot in both the caveolin-1 gene transfected MGC803 cells and the controls, The cell cycle was analyzed by flow cytometry. Results After transfected with caveolin-1, MGC803 cells significantly up-regulated the expression of caveolin 1 and extended their doubling time. The cell proliferation was inhibited and the cell cycle was arrested in the G0/G1 phase, Conclusion Caveolin-1 can inhibit the proliferation of MGC803 cells and induce cell cycle arrest in G0/G1 phase.

关 键 词：细胞质膜微囊蛋白 胃肿瘤 细胞增殖 

分 类 号：R735.2[医药卫生—肿瘤]