喉鳞状细胞癌中金属硫蛋白功能亚型的表达及意义  

Expression and significance of metallothionein isoforms in laryngeal squamous cell cancer

作　　者：赵胤[1] 梁春锋[2] 金春顺[1] 姚亚芬[3] 张素艳[4] 张彬[5] 

机构地区：[1]吉林大学第二医院耳鼻咽喉科,长春130041 [2]吉林大学第一医院耳鼻咽喉科,长春130021 [3]佳木斯大学第一附属医院耳鼻咽喉科,黑龙江佳木斯154003 [4]河北常山生化药业股份有限公司,石家庄050000 [5]石药集团恩必普药业有限公司,石家庄052160

出　　处：《中国肿瘤临床与康复》2006年第5期385-388,共4页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨金属硫蛋白(m etalloth ione ins,MT)各功能亚型在喉癌组织中的表达与喉癌临床病理生物学行为的关系。方法应用半定量RT-PCR方法,从分子水平研究MT各功能亚型在48例喉癌组织和15例正常黏膜组织的表达,分析MT各功能亚型与喉癌临床病理生物学行为的关系。结果48例喉癌组织中,在43例标本中MT-1 f、MT-1h和MT-2 a在喉癌组织显著高于12例正常组织中的表达(P<0.01),余8例为阴性表达,并且MT-1 f和MT-2 a在中、低分化喉癌组织的表达显著高于高分化组织(P<0.05),而MT-1 a在喉癌组织中表达减少(P<0.05),MT-1x在喉癌组织未见表达,但在正常组织中可见表达。结论MT-1 f和MT-2 a与喉癌分化程度显著相关,而MT-1 a可能与肿瘤细胞的凋亡有关。金属硫蛋白可能参与了喉癌细胞的增殖和凋亡,在喉癌的发生发展过程中起一定的作用。Objective To explore the expression of metallothioneins（MT） isoforms and the correlation of MT with clinicopathological behavior of laryngeal cancer. Methods Reverse transcription-polymerase chain reaction（RT-PCR） was used to examine expression of MT in 48 cases of laryngeal cancer and 15 cases of normal tissues. The relationship between MT isoforms and clinicopathological behavior of laryngeal cancer was analyzed. Results Expression of MT isoforms MT-1f, MT-1 h and MT-2a in 43 cases of laryngeal cancer was significantly higher than that in 12 cases of normal tissues （P 〈 0. 01 ）. The other cases were negative expression. MT-1f and MT-2a had a close relation with histological grades（ P 〈 0. 05 ）. But no significandy different expression of MT-lf and MT-2a was found in different clinical stages and primary locations of laryngeal cancer. Expression of MT-la decreased in laryngeal cancer. Expression of MT-lx was only found in normal tissues. Conclusions MT-1f and MT-2a have significant correlation with histological grades and cellular proliferation. MT-la is related to apoptosis. MT play an important role in the progression of laryngeal cancer.

关 键 词：金属硫蛋白 金属硫蛋白功能亚型 喉鳞状细胞癌 组织学分级 

分 类 号：R739.6[医药卫生—肿瘤]